BD Biosciences, San Jose, CA, USA.
Adv Exp Med Biol. 2019;1129:63-79. doi: 10.1007/978-981-13-6037-4_5.
In this review, we describe the BD Rhapsody™ Single-Cell Analysis System, a platform that allows high-throughput capture of nucleic acids from single cells using a simple cartridge workflow and a multitier barcoding system. The resulting captured information can be used to generate various types of next-generation sequencing (NGS) libraries, including whole transcriptome analysis for discovery biology and targeted RNA analysis for high sensitivity transcript detection. The BD Rhapsody system can be used with emerging applications, such as BD™ AbSeq assays, to profile gene expression in both mRNA and protein level to provide ultra-high resolution analysis of single cells.
在这篇综述中，我们描述了 BD Rhapsody™ 单细胞分析系统，该平台使用简单的试剂盒工作流程和多层条形码系统，实现了从单个细胞中高通量捕获核酸。所获得的捕获信息可用于生成各种类型的下一代测序 (NGS) 文库，包括用于发现生物学的全转录组分析和用于高灵敏度转录检测的靶向 RNA 分析。BD Rhapsody 系统可与新兴应用（如 BD™ AbSeq 测定法）结合使用，以在 mRNA 和蛋白质水平上对基因表达进行分析，从而对单个细胞进行超高分辨率分析。
