Beijing Advanced Innovation Center for Genomics (ICG), Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, College of Engineering, and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.
School of Life Sciences, and Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China.
Mol Cell. 2019 Jan 3;73(1):130-142.e5. doi: 10.1016/j.molcel.2018.10.020. Epub 2018 Nov 21.
Since its establishment in 2009, single-cell RNA sequencing (RNA-seq) has been a major driver behind progress in biomedical research. In developmental biology and stem cell studies, the ability to profile single cells confers particular benefits. Although most studies still focus on individual tissues or organs, the recent development of ultra-high-throughput single-cell RNA-seq has demonstrated potential power in characterizing more complex systems or even the entire body. However, although multiple ultra-high-throughput single-cell RNA-seq systems have attracted attention, no systematic comparison of these systems has been performed. Here, with the same cell line and bioinformatics pipeline, we developed directly comparable datasets for each of three widely used droplet-based ultra-high-throughput single-cell RNA-seq systems, inDrop, Drop-seq, and 10X Genomics Chromium. Although each system is capable of profiling single-cell transcriptomes, their detailed comparison revealed the distinguishing features and suitable applications for each system.
自 2009 年成立以来，单细胞 RNA 测序（RNA-seq）一直是推动生物医学研究进展的主要动力。在发育生物学和干细胞研究中，对单细胞进行分析的能力具有特殊的优势。尽管大多数研究仍集中在单个组织或器官上，但最近开发的超高通量单细胞 RNA-seq 已经展示了在描述更复杂系统甚至整个生物体方面的潜在能力。然而，尽管有多个超高通量单细胞 RNA-seq 系统引起了关注，但尚未对这些系统进行系统比较。在这里，我们使用相同的细胞系和生物信息学管道，为三种广泛使用的基于液滴的超高通量单细胞 RNA-seq 系统 inDrop、Drop-seq 和 10X Genomics Chromium 中的每一个开发了可直接比较的数据集。尽管每个系统都能够对单细胞转录组进行分析，但它们的详细比较揭示了每个系统的独特特征和适用范围。
