Aicher Toby P, Carroll Shaina, Raddi Gianmarco, Gierahn Todd, Wadsworth Marc H, Hughes Travis K, Love Chris, Shalek Alex K
Ragon Institute of MGH, Harvard, and MIT, Cambridge, MA, USA.
Department of Chemistry, Institute for Medical Engineering and Sciences (IMES), MIT, Cambridge, MA, USA.
Methods Mol Biol. 2019;1979:111-132. doi: 10.1007/978-1-4939-9240-9_8.
Seq-Well is a low-cost picowell platform that can be used to simultaneously profile the transcriptomes of thousands of cells from diverse, low input clinical samples. In Seq-Well, uniquely barcoded mRNA capture beads and cells are co-confined in picowells that are sealed using a semipermeable membrane, enabling efficient cell lysis and mRNA capture. The beads are subsequently removed and processed in parallel for sequencing, with each transcript's cell of origin determined via the unique barcodes. Due to its simplicity and portability, Seq-Well can be performed almost anywhere.
Seq-Well是一种低成本的皮升孔平台，可用于同时分析来自各种低输入量临床样本的数千个细胞的转录组。在Seq-Well中，带有独特条形码的mRNA捕获珠和细胞共同被限制在使用半透膜密封的皮升孔中，从而实现高效的细胞裂解和mRNA捕获。随后去除珠子并进行平行处理以进行测序，通过独特的条形码确定每个转录本的来源细胞。由于其简单性和便携性，Seq-Well几乎可以在任何地方进行。
