Department of Dermatology, St George Hospital, Sydney, New South Wales, Australia.
Faculty of Medicine, University of New South Wales, Sydney, New South Wales, Australia.
Australas J Dermatol. 2019 Aug;60(3):e173-e177. doi: 10.1111/ajd.13034. Epub 2019 Apr 9.
The BIOCHIP (Dermatology Mosaic 7, EUROIMMUN, Lubeck, Germany) is a novel multiplex indirect immunofluorescence technique used in the serological diagnosis of bullous pemphigoid. The BIOCHIP method combines the screening of several autoantibodies and target antigen-specific substrates in a single miniature incubation field to allow for simultaneous processing of the most common autoimmune bullous diseases autoantibodies using a single investigation. This manuscript reviews the literature on the validity of the BIOCHIP in the diagnosis of bullous pemphigoid. A systematic search for journal articles comparing the sensitivity and specificity of diagnostic tests for bullous pemphigoid patients was conducted in online databases via the Ovid SP search interfaces. The literature search generated 745 articles of which 189 were deemed relevant. Among the relevant articles, seven studies investigated the validity of the BIOCHIP indirect immunofluorescent test in the diagnosis of bullous pemphigoid. The BIOCHIP has demonstrated itself to be a specific test for BP180 (96.5-100%) and BP230 (98.3-100%), with a high sensitivity for BP180 (83.3-100%) but poor sensitivity for BP230 (24.3-66.7%). The BIOCHIP mosaic-based immunofluorescence test is potentially a simple, time- and effort-saving test that can aid in the diagnosis and screening of bullous pemphigoid. Further studies should report on the inter-rater reliability of the BIOCHIP to further validate the tool.
BIOCHIP（Dermatology Mosaic 7，EUROIMMUN，Lubeck，Germany）是一种新型的多重间接免疫荧光技术，用于大疱性类天疱疮的血清学诊断。BIOCHIP 方法将几种自身抗体和靶抗原特异性底物的筛选结合在单个微型孵育场中，允许使用单个检查同时处理最常见的自身免疫性大疱病自身抗体。本文综述了 BIOCHIP 在大疱性类天疱疮诊断中的有效性的文献。通过 Ovid SP 搜索界面在在线数据库中对比较大疱性类天疱疮患者诊断测试的敏感性和特异性的期刊文章进行了系统搜索。文献搜索产生了 745 篇文章，其中 189 篇被认为是相关的。在相关文章中，有七项研究调查了 BIOCHIP 间接免疫荧光试验在大疱性类天疱疮诊断中的有效性。BIOCHIP 已被证明是 BP180（96.5-100%）和 BP230（98.3-100%）的特异性测试，对 BP180 具有高敏感性（83.3-100%），但对 BP230 的敏感性较差（24.3-66.7%）。基于 BIOCHIP 马赛克的免疫荧光试验可能是一种简单、省时省力的测试，可以辅助大疱性类天疱疮的诊断和筛查。进一步的研究应该报告 BIOCHIP 的观察者间可靠性，以进一步验证该工具。
