Manousopoulou Antigoni, Al-Daghri Nasser M, Sabico Shaun, Garay-Baquero Diana J, Teng Jie, Alenad Amal, Alokail Majed S, Athanasopoulos Nikos, Deligeoroglou Efthymios, Chrousos George P, Bacopoulou Flora, Garbis Spiros D
Institute for Life Sciences, University of Southampton, Southampton, UK.
Chair for Biomarkers of Chronic Diseases, Biochemistry Department, King Saud University, Kingdom of Saudi Arabia.
Proteomics Clin Appl. 2019 Sep;13(5):e1800184. doi: 10.1002/prca.201800184. Epub 2019 Apr 23.
Polycystic ovary syndrome (PCOS) is a common endocrine disorder associated with insulin resistance, even in the absence of overweight/obesity. The aim of the present study is to examine the global serum proteomic profile of adolescent, normal-weight females with PCOS in order to gain novel insight in the association of this endocrine disorder with insulin physiology and to identify novel circulating markers that can guide intervention protocols.
Non-depleted serum from normal-weight (BMI: 18-23 kg m ), adolescent females (13-21 years old) with PCOS (n = 20) is compared to BMI- and age-matched healthy controls (n = 20) using our 3D quantitative proteomics methodology. Serum samples from study participants are randomly pooled to form four biological replicates of females with PCOS and four of healthy controls (n = 5 per sample pool).
One-hundred and twenty-six proteins are differentially expressed in females with PCOS compared to controls. Gene ontology analysis shows significant enrichment for terms related to inflammatory immune response, metabolism and insulin-like growth factor receptor signaling pathway. Circulating levels of IGF-1 and -2 and IGFBP-2, -3, and -4 are found to be lower in females with PCOS compared to healthy controls.
The present serum proteomics study provides insight into the pro-inflammatory status and insulin dysregulation in young females with PCOS and identifies potential serological markers that can guide early intervention protocols.
多囊卵巢综合征（PCOS）是一种常见的内分泌紊乱疾病，即使在无超重/肥胖的情况下也与胰岛素抵抗相关。本研究的目的是检测青春期体重正常的PCOS女性的血清蛋白质组学概况，以便深入了解这种内分泌紊乱与胰岛素生理之间的关联，并识别可指导干预方案的新型循环标志物。
使用我们的三维定量蛋白质组学方法，将体重正常（BMI：18 - 23 kg/m²）、年龄在13 - 21岁的青春期PCOS女性（n = 20）的非耗尽血清与BMI和年龄匹配的健康对照（n = 20）进行比较。研究参与者的血清样本被随机合并，形成4个PCOS女性的生物学重复样本和4个健康对照的生物学重复样本（每个样本池n = 5）。
与对照组相比，126种蛋白质在PCOS女性中差异表达。基因本体分析显示，与炎症免疫反应、代谢和胰岛素样生长因子受体信号通路相关的术语显著富集。与健康对照相比，PCOS女性中IGF - 1、IGF - 2以及IGFBP - 2、IGFBP - 3和IGFBP - 4的循环水平较低。
本血清蛋白质组学研究深入了解了年轻PCOS女性的促炎状态和胰岛素失调情况，并识别出了可指导早期干预方案的潜在血清学标志物。
