Molnar J A, Cunningham J J, Miyatani S, Vizulis A, Wright J D, Burke J F
J Appl Physiol (1985). 1986 Oct;61(4):1582-5. doi: 10.1152/jappl.1986.61.4.1582.
A closed-circuit metabolic system has been designed and tested for multiple applications. Air pressure within a closed chamber is regulated electronically while allowing for respiratory gas exchange. Compared with a previously reported standard indirect calorimetry system, the new device had by virtue of longer duration of measurement improved precision (coefficient of variation 3% vs. 14%) during studies of O2 consumption both at room temperature and at 5 degrees C. In addition, a more physiological atmospheric environment is maintained. This system has also been utilized for simultaneously labeling groups of up to 20 weanling rats with 18O2 over a 2-day period and for exposure of rats to a hyperoxic (84% O2), normobaric environment for 4-day periods. Potential applications include maintenance of pressure (hypobaric through hyperbaric) and O2 (hypoxic through hyperoxic) controlled environments, exposure to toxic gases, study of diurnal variations in metabolic rate, measurement of metabolic expenditure with activity, and adaptation to other species including humans.
已设计并测试了一种用于多种应用的闭路代谢系统。封闭室内的气压通过电子方式调节，同时允许呼吸气体交换。与先前报道的标准间接量热法系统相比，在室温和5摄氏度下研究氧气消耗时，新设备由于测量持续时间更长，精度有所提高（变异系数分别为3%和14%）。此外，还能维持更接近生理状态的大气环境。该系统还被用于在两天时间内同时用18O2标记多达20只断奶大鼠，并将大鼠置于高氧（84% O2）、常压环境中4天。潜在应用包括维持压力（从低压到高压）和氧气（从低氧到高氧）可控环境、暴露于有毒气体、研究代谢率的昼夜变化、测量活动时的代谢消耗以及适用于包括人类在内的其他物种。
