五苯甲麦芽糖的自组装行为及其在膜蛋白研究中的应用。
Self-Assembly Behaviors of a Penta-Phenylene Maltoside and Its Application for Membrane Protein Study.
机构信息
Department of Bionanotechnology, Hanyang University, Ansan, 15588, Korea.
Current address: Department of Chemistry, Mirpur University of Science&Technology (MUST), Mirpur-, 10250 (AJK), Pakistan.
出版信息
Chem Asian J. 2019 Jun 3;14(11):1926-1931. doi: 10.1002/asia.201900224. Epub 2019 Apr 10.
Abstract
We prepared an amphiphile with a penta-phenylene lipophilic group and a branched trimaltoside head group. This new agent, designated penta-phenylene maltoside (PPM), showed a marked tendency to self-assembly into micelles via strong aromatic-aromatic interactions in aqueous media, as evidenced by H NMR spectroscopy and fluorescence studies. When utilized for membrane protein studies, this new agent was superior to DDM, a gold standard conventional detergent, in stabilizing multiple proteins long term. The ability of this agent to form aromatic-aromatic interactions is likely responsible for enhanced protein stabilization when associated with a target membrane protein.
摘要
我们制备了一种具有五苯脂溶性基团和支化三麦芽苷头部基团的两亲分子。这种新的试剂被命名为五苯麦芽苷（PPM），在水溶液中通过强芳香-芳香相互作用显示出明显的自组装成胶束的趋势，这一点可以通过 H NMR 光谱和荧光研究得到证明。在用于膜蛋白研究时，这种新试剂在长期稳定多种蛋白质方面优于 DDM，这是一种标准的传统去污剂。当与靶膜蛋白结合时，该试剂形成芳香-芳香相互作用的能力可能是增强蛋白质稳定性的原因。
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