Claassen P A, Kortstee G J, Oosterveld-van Vliet W M, van Neerven A R
J Bacteriol. 1986 Nov;168(2):791-4. doi: 10.1128/jb.168.2.791-794.1986.
In acetate-limited chemostat cultures started with single-colony cultures of Thiobacillus versutus, a mutant appeared after approximately 85 volume changes. The inhomogeneity of the culture was detected by the development of two different types of colonies on agar plates. When a pure culture of the mutant was grown in a chemostat, parent colonies appeared after almost the same period of time. Electron micrographs of the mutant grown on butyrate showed the presence of fibrils surrounding the cells. The cells of the parent strain were bald when grown under the same conditions. The growth kinetics of the parent and the mutant were investigated in batch cultures with a variety of substrates and were found to be identical. Major differences between the two strains were observed during growth on mannitol; the mutant attained a lower yield and excreted large amounts of extracellular polysaccharides.
在以维氏硫杆菌单菌落培养物起始的乙酸盐限制恒化器培养中，大约经过85倍体积变化后出现了一个突变体。通过在琼脂平板上形成两种不同类型的菌落检测到培养物的不均匀性。当突变体的纯培养物在恒化器中生长时，几乎在相同时间段后出现了亲代菌落。在丁酸盐上生长的突变体的电子显微照片显示细胞周围存在纤丝。在相同条件下生长时，亲代菌株的细胞是光秃的。在分批培养中用多种底物研究了亲代和突变体的生长动力学，发现它们是相同的。在甘露醇上生长期间观察到这两种菌株之间的主要差异；突变体的产量较低，并分泌大量细胞外多糖。
