Szmitkowski M, Prokopowicz J
Acta Haematol Pol. 1978 Aug-Sep;9(3):165-8.
Activity of CSF was measured in human serum after chromatography on Sephadex G-200. Agar culture of bone marrow cells was done in Petri dishes with agar in Eagle'a medium containing fetal calf serum and antibiotics. This agar-medium containing mouse bone marrow cells, was allowed to gel and the dishes were incubated at 37 degrees C in a fully humidified atmosphere of 10% CO2 in air. Our data indicate that chromatography procedure allows to get CSF free from its inhibitors. All activity was localized in one peak, in the region of low molecular weight proteins.
在Sephadex G - 200上进行色谱分析后，测定人血清中CSF的活性。在含有胎牛血清和抗生素的伊格尔培养基中，于培养皿中用琼脂对骨髓细胞进行琼脂培养。将含有小鼠骨髓细胞的这种琼脂培养基使其凝固，然后将培养皿在37℃、空气含10%二氧化碳的完全湿润的气氛中孵育。我们的数据表明，色谱分析程序能够获得不含抑制剂的CSF。所有活性都集中在一个峰中，位于低分子量蛋白质区域。
