Entringer M A, Robinson W A, Drebing C J
Exp Hematol. 1980 Nov;8(10):1232-40.
Two hundred and thirty normal human sera have been tested for colony stimulating factor (CSF) under carefully controlled conditions using human bone marrow and the semi-solid agar-gel technique. It has been shown that CSF can be detected in most sera when incorporated into underlayers to remove potential inhibitors. The effect of storage temperature on serum CSF has been determined. Storage at 4 degrees, --15 degrees, and --66 degrees for up to 80 days resulted in only a mild decrease in CSF levels. After 240 days of storage, CSF values fell to 50% of that found before storage. Repeated freeze-thawing of serum has not been shown to decrease CSF levels when done in Pyrex glassware. These studies will serve as a baseline for those wishing to study human serum CSA in hematopoietic disorders.
在精心控制的条件下，使用人骨髓和半固体琼脂凝胶技术，对230份正常人血清进行了集落刺激因子（CSF）检测。结果表明，当将血清加入底层以去除潜在抑制剂时，大多数血清中均可检测到CSF。已确定储存温度对血清CSF的影响。在4℃、-15℃和-66℃储存长达80天，CSF水平仅轻微下降。储存240天后，CSF值降至储存前的50%。在派热克斯玻璃器皿中进行血清反复冻融，未显示CSF水平降低。这些研究将为那些希望研究造血系统疾病中人血清CSA的人提供一个基线。
