从猪白细胞培养物中鉴定出两种粒细胞/巨噬细胞集落刺激因子。

Identification of two granulocyte/macrophage colony-stimulating factors from porcine leukocyte cultures.

作者信息

Neumeier R, Maurer H R, Arnold M, Gerlach U, Glendinning K, Renner H, Wissler J H

出版信息

Hoppe Seylers Z Physiol Chem. 1982 Feb;363(2):193-5.

Abstract

Granulocyte/macrophage colony-stimulating factors (CSF) were found in large-scale serum-free cultures of porcine blood leukocytes. The factors were concentrated from the conditioned culture medium of Concanavalin A-stimulated leukocytes using ammonium sulfate salting-out fractionation. The protein precipitate at 45-90% salt saturation contained two CSF types upon gel filtration on Sephadex G-100: One type was found to be active on mouse, but not on human bone marrow stem cells and had an apparent molecular mass of 85 000-100 000 Da; the other of about 40 000-45 000 Da stimulated both mouse and human bone marrow stem cells.

摘要

在猪血液白细胞的大规模无血清培养物中发现了粒细胞/巨噬细胞集落刺激因子（CSF）。使用硫酸铵盐析分级分离法从伴刀豆球蛋白A刺激的白细胞的条件培养基中浓缩这些因子。在45%-90%盐饱和度下的蛋白质沉淀物在Sephadex G-100上进行凝胶过滤时含有两种CSF类型：一种类型对小鼠有活性，但对人骨髓干细胞无活性，表观分子量为85 000-100 000 Da；另一种约40 000-45 000 Da的类型对小鼠和人骨髓干细胞均有刺激作用。