Costa Altair da Silva, Miotto Andre, Paulo Gustavo Andrade de, Ferrari Angelo Paulo, Ota Luiz Hirotoshi
Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
Einstein (Sao Paulo). 2019 Apr 8;17(2):eAO4533. doi: 10.31744/einstein_journal/2019AO4533.
To evaluate the feasibility and applicability of a low-cost cryotherapy system.
Experimental study with 25kg Landrace pigs submitted to a longitudinal cervico-thoraco-abdominal incision for exposure of the trachea, thorax and abdomen. The tissues were frozen by continuous spray application at different periods of time (5, 10 and 15 seconds). Spray cryotherapy was performed using a fluorinated gas (tetrafluorethane) delivered at - 47°C temperature (DermaFreeze®, Emdutos; ANVISA registration 80409950001; price R$ 394,00). via an adapted, disposable 1.8mm cholangiography catheter (Olympus; price R$ 280,00). The specimens were resected for histopathological analysis.
Thirty samples were obtained from ten different organs and divided according to spray cryotherapy application time. System activation for 5, 10 or 15 seconds led to consumption of 14g, 27g and 40g of gas respectively (average gas consumption, 2.7g/s using a 1.8mm catheter). The system comprising a spray tube and catheter proved user-friendly and effective, with constant gas dispersion and adequate tissue freezing. In spite of effective freezing, microscopy failed to reveal tissue changes. This may have reflected methodological constraints precluding evaluation at tissue damage peak time (48 hours).
The low-cost spray cryotherapy system proved feasible and safe.
评估一种低成本冷冻治疗系统的可行性和适用性。
对25千克的长白猪进行实验研究，沿颈胸腹做纵向切口以暴露气管、胸部和腹部。在不同时间段（5、10和15秒）通过持续喷雾对组织进行冷冻。使用在-47°C温度下输送的氟化气体（四氟乙烷）（DermaFreeze®，Emdutos；巴西国家卫生监督局注册号80409950001；价格394.00雷亚尔），通过一根适配的一次性1.8毫米胆管造影导管（奥林巴斯；价格280.00雷亚尔）进行喷雾冷冻治疗。切除标本进行组织病理学分析。
从十个不同器官获取了30个样本，并根据喷雾冷冻治疗的应用时间进行划分。系统激活5、10或15秒分别导致消耗14克、27克和40克气体（使用1.8毫米导管时平均气体消耗量为2.7克/秒）。由喷雾管和导管组成的系统证明使用方便且有效，气体分散均匀，组织冷冻充分。尽管冷冻有效，但显微镜检查未发现组织变化。这可能反映了方法上的限制，无法在组织损伤峰值时间（48小时）进行评估。
低成本喷雾冷冻治疗系统证明是可行且安全的。
