Department of Pharmaceutical Microbiology, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, China.
Department of Pharmaceutical Microbiology, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, China.
Fish Shellfish Immunol. 2019 Jun;89:319-325. doi: 10.1016/j.fsi.2019.04.014. Epub 2019 Apr 7.
The objective of this study was to investigate the effect of dietary quorum quenching bacterium Bacillus cereus QSI-1 on skin mucus protein pattern and innate immune response in Crucian Carp (Carassius auratus gibelio). The differential proteomes of skin mucus of Crucian Carp were analyzed after administration of Bacillus cereus QSI-1 by isobaric tags for relative and absolute quantitation (iTRAQ) labeling, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 1974 proteins were quantified. Using a 1.5-fold change in expression as a physiological significant benchmark, 264 differentially expressed proteins were reliably quantified by iTRAQ analysis, including 130 up- and 134 down-regulated proteins after dietary Bacillus cereus QSI-1. Some Proteins that were involved in immunity included protein S100, annexin, histone H3, lymphocyte cytosolic protein 1, heat shock protein, L-plastin, keratin 91, etc. Furthermore, fish fed 5 × 10 CFU/g Bacillus cereus QSI-1 supplemented diet showed an increase in alternative complement activity and lysozyme activity but expressed a decrease in superoxide dismutase activity in skin mucus (P < 0.05). However, administration of Bacillus cereus QSI-1 had no significant effects on total immunoglobulin level (P > 0.05). These results demonstrated that dietary administration of Bacillus cereus QSI-1 affects skin mucus protein profile and innate immune response in Crucian Carp, and also can enhance the disease resistance of Crucian Carp against A. hydrophila. This is the first report on proteomics analysis of skin mucus proteins in Crucian Carp after administration of quorum quenching bacterium Bacillus cereus, and the results will help to understand the mucosal immune responses to probiotics at the protein level in fish.
本研究旨在探讨日粮中添加生防蜡样芽胞杆菌 QSI-1 对异育银鲫皮肤黏液蛋白图谱和固有免疫应答的影响。采用相对和绝对定量同位素标记(iTRAQ)标记和液相色谱-串联质谱(LC-MS/MS)技术分析异育银鲫皮肤黏液的差异蛋白质组。共定量了 1974 种蛋白质。使用表达变化 1.5 倍作为生理显著基准，通过 iTRAQ 分析可靠地定量了 264 种差异表达蛋白，包括日粮添加生防蜡样芽胞杆菌 QSI-1 后上调的 130 种蛋白和下调的 134 种蛋白。一些参与免疫的蛋白质包括 S100 蛋白、膜联蛋白、组蛋白 H3、淋巴细胞胞浆蛋白 1、热休克蛋白、L- 肌动蛋白、角蛋白 91 等。此外，投喂 5×10 CFU/g 生防蜡样芽胞杆菌 QSI-1 日粮的鱼皮肤黏液中的替代补体活性和溶菌酶活性增加，但超氧化物歧化酶活性降低（P<0.05）。然而，日粮添加生防蜡样芽胞杆菌 QSI-1 对总免疫球蛋白水平没有显著影响（P>0.05）。这些结果表明，日粮中添加生防蜡样芽胞杆菌 QSI-1 影响异育银鲫皮肤黏液蛋白图谱和固有免疫应答，并能增强异育银鲫对嗜水气单胞菌的抗病能力。这是首次报道日粮添加生防蜡样芽胞杆菌后异育银鲫皮肤黏液蛋白质组学分析的研究，结果将有助于了解鱼类益生菌的黏膜免疫应答在蛋白质水平上的变化。
