保存温度对淋病奈瑟菌分离的影响。
Effect of holding temperature on isolation of Neisseria gonorrhoeae.
作者信息
Evans K D, Peterson E M, Curry J I, Greenwood J R, de la Maza L M
出版信息
J Clin Microbiol. 1986 Dec;24(6):1109-10. doi: 10.1128/jcm.24.6.1109-1110.1986.
The effect of holding temperature on the recovery of Neisseria gonorrhoeae was studied. From 300 specimens tested, Thayer-Martin medium plates inoculated and incubated in the presence of CO2 at 35, 22, and 4 degrees C for 24 h before incubation at 35 degrees C yielded 100, 96, and 95% of all isolates ultimately recovered from 82 positive specimens. Although there was a decrease in the quantity of organisms recovered, initial incubation of specimens under refrigeration or at room temperature yielded greater than or equal to 95% of the positive specimens.
研究了保存温度对淋病奈瑟菌复苏的影响。在300份检测标本中，接种于Thayer-Martin培养基平板上，并在二氧化碳环境下分别于35℃、22℃和4℃孵育24小时，然后在35℃孵育，最终从82份阳性标本中分离出的所有菌株中，分别有100%、96%和95%被检出。尽管复苏的菌量有所减少，但标本在冷藏或室温下进行初始孵育后，阳性标本的检出率大于或等于95%。
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