Jacobs N F, Kraus S J
J Clin Microbiol. 1975 May;1(5):401-4. doi: 10.1128/jcm.1.5.401-404.1975.
Translucent culture media are essential for the determination of gonococcal colony types. In this study two hemoglobin-free, translucent culture media were compared with Thayer-Martin (TM) medium for the primary isolation of Neisseria gonorrhoeae from male urethral specimens. The antibiotic-free translucent medium (GCB) and the translucent medium containing vancomycin, colistin, and nystatin (VCN) were as efficient as TM medium in detecting positive specimens. In fact, VCN medium gave positive results significantly more often than TM medium(P less than 0.05). The amount of growth on positive plates was comparable for all three media. Contaminants were noted most often on GCB medium (P less than 0.0001), and the frequency of contaminants with TM plates exceeded that of VCN plates (P less than 0.0001). The high isolation rate of N. gonorrhoeae with VCN hemoglobin-free culture medium and its lower cost and greater ease of preparation make it a suitable alternative to TM medium for use with male urethral specimens.
半透明培养基对于淋病奈瑟菌菌落类型的鉴定至关重要。在本研究中，将两种无血红蛋白的半透明培养基与泰-马（TM）培养基进行比较，用于从男性尿道标本中初次分离淋病奈瑟菌。无抗生素的半透明培养基（GCB）和含有万古霉素、多粘菌素和制霉菌素的半透明培养基（VCN）在检测阳性标本方面与TM培养基一样有效。事实上，VCN培养基产生阳性结果的频率显著高于TM培养基（P小于0.05）。三种培养基在阳性平板上的生长量相当。在GCB培养基上最常发现污染物（P小于0.0001），TM平板上的污染物频率超过VCN平板（P小于0.0001）。VCN无血红蛋白培养基对淋病奈瑟菌的高分离率及其较低的成本和更高的制备简易性使其成为与男性尿道标本一起使用时TM培养基的合适替代品。
