Liu Yanqiu, Ding Yaqi, Ye Min, Zhu Tao, Tian Danbi, Ding Kan
Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, China, University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China.
Nano Science and Technology Institute, University of Science and Technology of China, 96 Jin Zhai Road, Hefei 230026, China.
Polymers (Basel). 2017 Jun 16;9(6):228. doi: 10.3390/polym9060228.
A heterogalactan, named ACW0, was extracted from and purified by anion exchange and gel permeation chromatography. It was composed of galactose (94.98%), traces of mannose (2.41%), and fucose (2.61%), with its molecular weight estimated to be 13.5 k Da. The polysaccharide ACW0 was shown to be a mannofucogalactan with a backbone chain of α-d-1,6-linked Gal, attached by a non-reducing terminal α-d-Man and α-l-Fuc on C-2 of nearly every six α-d-1,6-linked Gal residues. A sulfated polysaccharide, ACW0-Sul was achieved by the chlorosulfonic acid-pyridine method. Compared with the native polysaccharide, ACW0-Sul could disrupt tube formation and migration as well as cell growth of human microvascular endothelial cells (HMEC-1) dose-dependently. Further studies revealed that phosphorylation of Extracellular Regulated Protein Kinases (Erk) and Focal Adhesion Kinase (FAK) were significantly inhibited by ACW0-Sul. These results suggested that ACW0-Sul could be a potent candidate for anti-angiogenic agent development.
从[具体来源未提及]中提取出一种名为ACW0的杂半乳聚糖，并通过阴离子交换和凝胶渗透色谱法进行纯化。它由半乳糖（94.98%）、微量甘露糖（2.41%）和岩藻糖（2.61%）组成，其分子量估计为13.5 kDa。多糖ACW0被证明是一种甘露糖岩藻半乳聚糖，其主链为α-d-1,6-连接的半乳糖，几乎每六个α-d-1,6-连接的半乳糖残基在C-2位通过非还原末端的α-d-甘露糖和α-l-岩藻糖连接。通过氯磺酸-吡啶法获得了一种硫酸化多糖ACW0-Sul。与天然多糖相比，ACW0-Sul可以一种硫酸化后能够剂量依赖性地破坏人微血管内皮细胞（HMEC-1）的管腔形成、迁移以及细胞生长。进一步研究表明，ACW0-Sul能显著抑制细胞外调节蛋白激酶（Erk）和粘着斑激酶（FAK）的磷酸化。这些结果表明，ACW0-Sul可能是抗血管生成药物开发的有力候选物。
