School of Pharmacy, Zunyi Medical University, 201 Dalian Road, Zunyi 563003, PR China; Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, PR China.
Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, PR China.
Int J Biol Macromol. 2018 Mar;108:47-55. doi: 10.1016/j.ijbiomac.2017.11.111. Epub 2017 Nov 22.
Angiogenesis plays a crucial role in tumor growth and development. Blocking angiogenesis of tumor cells has become one of the most promising approaches in cancer therapy. Here, an acidic polysaccharide designated LBP1B-S-2 with an average molecular weight of 80.00kDa, was extracted and purified from dried mature fruits of Lycium barbarum L. by DEAE Sepharose™ Fast Flow and Sephacryl S-300 HR columns. Monosaccharide composition analysis indicated that the LBP1B-S-2 was composed of rhamnose, arabinose, galactose and glucuronic acid in a molar ratio of 3.13: 53.55: 39.37: 3.95. The backbone of LBP1B-S-2 was consisted of 1, 3-linked β-d-Galp, 1, 6-linked β-d-Galp and branches contained 1, 4-linked β-d-GlcpA, T-linked β-d-Galp, 1, 6-linked β-d-Galp, T-linked α-l-Araf, T-linked β-l-Araf, 1, 5-linked α-l-Araf and T-linked β-l-Rhap directly or indirectly attached to C-3 position of 1, 6-linked β-d-Galp or C-6 position of 1, 3-linked β-d-Galp, according to the results of partial acid hydrolysis analysis, methylation analysis, IR and NMR spectra. The sulfated derivative of LBP1B-S-2, could significantly inhibit the tube formation of human microvascular endothelial cells (HMEC-1) in vitro at concentration of (95nM) without significant cytotoxicity.
血管生成在肿瘤生长和发展中起着至关重要的作用。阻断肿瘤细胞的血管生成已成为癌症治疗中最有前途的方法之一。在这里，从枸杞的干成熟果实中提取并通过 DEAE Sepharose™ Fast Flow 和 Sephacryl S-300 HR 柱纯化得到一种酸性多糖，命名为 LBP1B-S-2，其平均分子量为 80.00kDa。单糖组成分析表明，LBP1B-S-2 由鼠李糖、阿拉伯糖、半乳糖和葡萄糖醛酸组成，摩尔比为 3.13:53.55:39.37:3.95。LBP1B-S-2 的骨架由 1,3-连接的β-d-Galp、1,6-连接的β-d-Galp 和支链组成，支链含有 1,4-连接的β-d-GlcpA、T-连接的β-d-Galp、1,6-连接的β-d-Galp、T-连接的α-l-Araf、T-连接的β-l-Araf、1,5-连接的α-l-Araf 和 T-连接的β-l-Rhap，这些糖直接或间接连接到 1,6-连接的β-d-Galp 的 C-3 位或 1,3-连接的β-d-Galp 的 C-6 位，根据部分酸水解分析、甲基化分析、IR 和 NMR 光谱的结果。LBP1B-S-2 的硫酸化衍生物在浓度为（95nM）时，可显著抑制体外人微血管内皮细胞（HMEC-1）的管形成，而无明显细胞毒性。
