Leo O, Sachs D H, Samelson L E, Foo M, Quinones R, Gress R, Bluestone J A
J Immunol. 1986 Dec 15;137(12):3874-80.
Monoclonal antibodies (mAb) directed at the T cell receptor complex (TcR) on cloned T cells have generally been identified by their ability to inhibit the clone's antigen-specific function. Because such inhibition is highly dependent on antibody concentration and affinity, detection of anti-clonotypic antibodies to murine alloreactive T cells has been very difficult. In this report, an alternative method is described on the basis of the ability of antibodies specific for the TcR complex to activate T cells in an antigen-independent manner. The assay is based upon the observation that soluble antibodies to human T3 promote lysis of irrelevant, Fc receptor-positive targets by a human CTL line. By using this approach, an anti-TcR mAb has been identified among a panel of murine mAb generated against an alloreactive CTL clone. Induction of lysis by soluble anti-TcR mAb has been shown to require both the expression of Fc receptors on the target cell and conjugate formation between the effector and the target cell. This assay provides a screening procedure that is much more sensitive than inhibition of function, and it preferentially detects antibodies specific for cell surface molecules involved in T cell activation.
针对克隆化T细胞上的T细胞受体复合物（TcR）的单克隆抗体（mAb），通常是根据其抑制克隆的抗原特异性功能的能力来鉴定的。由于这种抑制高度依赖于抗体浓度和亲和力，因此检测针对小鼠同种异体反应性T细胞的抗独特型抗体一直非常困难。在本报告中，基于针对TcR复合物的特异性抗体以抗原非依赖性方式激活T细胞的能力，描述了一种替代方法。该检测基于这样的观察结果：针对人T3的可溶性抗体可促进人CTL系对无关的、Fc受体阳性靶标的裂解。通过使用这种方法，在针对同种异体反应性CTL克隆产生的一组鼠源mAb中鉴定出了一种抗TcR mAb。已证明可溶性抗TcR mAb诱导裂解既需要靶细胞上Fc受体的表达，也需要效应细胞与靶细胞之间形成共轭体。该检测提供了一种比功能抑制更灵敏的筛选程序，并且它优先检测针对参与T细胞激活的细胞表面分子的特异性抗体。
