骨钙素的酶免疫测定法。

An enzyme immunoassay for osteocalcin.

作者信息

Tanaka H, Kuwada M, Shiraki M, Katayama K

出版信息

J Immunol Methods. 1986 Nov 20;94(1-2):19-24. doi: 10.1016/0022-1759(86)90210-3.

Abstract

A sensitive and specific enzyme immunoassay for bovine osteocalcin was developed with the use of enzyme-labeled antigen. Bovine osteocalcin was conjugated with beta-D-galactosidase for use in the immunoassay. The minimum amount of osteocalcin detectable by this method was 5-10 pg/assay. The enzyme immunoassay could detect immunoreactive osteocalcin levels in normal human sera, giving a value of 6.58 +/- 0.72 ng/ml (mean +/- SE of 16 human subjects). The correlation coefficient between the levels measured by this method and radioimmunoassay was 0.94.

摘要

利用酶标记抗原开发了一种灵敏且特异的牛骨钙素酶免疫测定法。牛骨钙素与β-D-半乳糖苷酶结合用于免疫测定。该方法可检测到的骨钙素最小量为5 - 10 pg/测定。该酶免疫测定法可检测正常人血清中免疫反应性骨钙素水平，16名受试者的平均值为6.58 +/- 0.72 ng/ml（平均值 +/- 标准误）。该方法与放射免疫测定法所测水平之间的相关系数为0.94。