新型逆行充填材料对体外成骨细胞分化的影响。
Effects of newly-developed retrograde filling material on osteoblastic differentiation in vitro.
机构信息
Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University.
Department of Conservative Dentistry, School of Dentistry, Kyung Hee University.
出版信息
Dent Mater J. 2019 Jul 31;38(4):528-533. doi: 10.4012/dmj.2018-124. Epub 2019 Apr 9.
The aim of this study was to compare chemical properties and bioactivities of with mineral trioxide aggregate (MTA) and EndoSequence Root Repair Material (ERRM). After setting, surfaces of test materials were observed by scanning electron microscope (SEM). The pH and cell viability of materials were tested. Osteoblastic differentiation and alkaline phosphatase (ALP) activity were measured by quantitative real-time PCR and ALP staining. MTA showed spindle-shaped crystals while ERRM showed round-shaped crystals of various sizes. ERRM presented lower pH than MTA. Both materials showed good cell viabilities compared to the control. Expression levels of osteoblastic genes and ALP staining were increased significantly (p<0.05) in ERRM and MTA groups compared to those in the control group. In conclusion, ERRM and MTA both had effects on osteoblastic differentiation. Therefore, ERRM can be used as a desirable alternative to MTA for root-end filling material.
本研究旨在比较矿物三氧化物聚合体（MTA）和 EndoSequence 根管修复材料（ERRM）的化学性质和生物活性。凝固后，通过扫描电子显微镜（SEM）观察测试材料的表面。测试材料的 pH 值和细胞活力。通过定量实时 PCR 和碱性磷酸酶（ALP）染色测量成骨细胞分化和碱性磷酸酶（ALP）活性。MTA 显示出梭形晶体，而 ERRM 显示出各种大小的圆形晶体。ERRM 的 pH 值低于 MTA。与对照组相比，两种材料的细胞活力均较好。与对照组相比，ERRM 和 MTA 组成骨细胞基因的表达水平和 ALP 染色均显著增加（p<0.05）。总之，ERRM 和 MTA 均对成骨细胞分化有影响。因此，ERRM 可用作 MTA 的理想替代物，作为根管填充材料。
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