Department of Conservative Dentistry, College of Dentistry, University of Sulaimani, Sulaimani-Dream Land B-25, Kurdistan Region, Iraq.
Department of Oral Diagnosis, Oral and Maxillofacial Medicine in Kurdistan Board of Dental Specialties, College of Dentistry, University of Sulaimani, Kurdistan Region, Iraq.
Biomed Res Int. 2020 May 22;2020:9576930. doi: 10.1155/2020/9576930. eCollection 2020.
This study evaluated the bioactivity and physicochemical properties of three commercial calcium silicate-based endodontic materials (MTA, EndoSequence Root Repair Material putty, and Biodentine™). . Horizontal sections of 3 mm thickness from 18 root canals of human teeth were subjected to biomechanical preparation with WaveOne Gold large rotary instruments. The twelve specimens were filled with three tested materials (MM-MTA, EndoSequence Root Repair Material putty, and Biodentine™) and immersed in phosphate-buffered saline for 7 and 30 days. After this period of time, each specimen of each material was processed for morphological observation, surface precipitates, and interfacial dentin using SEM. In addition, the surface morphology of the set materials, without soaking in phosphate-buffered solution after one day and after 28 days stored in phosphate-buffered saline, was evaluated using SEM; also, the pH of the soaking water and the amount of calcium ions released from the test materials were measured by using an inductively coupled plasma-optical emission spectroscopy test. Data obtained were analyzed using one-way analysis of variance and Tukey's honest significant difference test with a significance level of 5%.
The formation of precipitates was observed on the surfaces of all materials at 1 week and increased substantially over time. Interfacial layers in some areas of the dentin-cement interface were found from one week of immersion. All the analyzed materials showed alkaline pH and capacity to release calcium ions; however, the concentrations of released calcium ions were significantly more in Biodentine and ESRRM putty than MM-MTA ( < 0.05). ESRRM putty maintained a pH of around 11 after 28 days.
Compared with MM-MTA, Biodentine and ESRRM putty showed significantly more calcium ion release. However, exposure of three tested cements to phosphate-buffered solution resulted in precipitation of apatite crystalline structures over both cement and dentin that increased over time. This suggests that the tested materials are bioactive.
本研究评估了三种商业硅酸钙基根管充填材料（MTA、EndoSequence Root Repair 材料糊剂和 Biodentine™）的生物活性和物理化学性能。方法：从 18 个人类牙齿的根管中制备厚度为 3mm 的水平切片，用 WaveOne Gold 大螺旋器械进行生物力学预备。将 12 个样本分别用三种测试材料（MM-MTA、EndoSequence Root Repair 材料糊剂和 Biodentine™）充填，并浸入磷酸盐缓冲液中 7 和 30 天。经过这段时间后，用 SEM 对每种材料的每个样本进行形态观察、表面沉淀物和界面牙本质的处理。此外，在不浸泡于磷酸盐缓冲溶液的情况下，用 SEM 评估了 1 天和 28 天后储存在磷酸盐缓冲液中的已凝固材料的表面形态，还通过电感耦合等离子体发射光谱法测量了浸泡水中的 pH 值和测试材料释放的钙离子量。采用单因素方差分析和 Tukey 氏显著差异检验对数据进行分析，检验水准为 5%。结果：在所有材料的表面在第 1 周观察到沉淀物的形成，并且随着时间的推移显著增加。在浸泡 1 周后，在牙本质-水泥界面的一些区域发现了界面层。所有分析的材料均呈碱性 pH 值，并具有释放钙离子的能力；然而，Biodentine 和 ESRRM 糊剂释放的钙离子浓度明显高于 MM-MTA（<0.05）。ESRRM 糊剂在 28 天后仍保持 pH 值约 11。结论：与 MM-MTA 相比，Biodentine 和 ESRRM 糊剂释放的钙离子明显更多。然而，三种测试水泥暴露于磷酸盐缓冲溶液中会导致在水泥和牙本质上沉淀出磷灰石结晶结构，并且随着时间的推移而增加。这表明测试材料具有生物活性。
