Ro/SSA自身抗原作为一种免疫原。一些抗Ro/SSA抗体与IgG结合。
The Ro/SSA autoantigen as an immunogen. Some anti-Ro/SSA antibody binds IgG.
作者信息
Mamula M J, Fox O F, Yamagata H, Harley J B
出版信息
J Exp Med. 1986 Dec 1;164(6):1889-901. doi: 10.1084/jem.164.6.1889.
Abstract
The rheumatic disease autoantigen, Ro/SSA, was immunogenic to a rabbit host. The heteroimmune rabbit serum bound the Ro/SSA particle in immunoblots and in an ELISA. Both the rabbit anti-Ro/SSA and a human prototype anti-Ro/SSA serum also bound IgG; and moreover, IgG inhibited both rabbit and human anti-Ro/SSA activity. Anti-IgG activity of the rabbit and human anti-Ro/SSA sera bound Ro/SSA by Western blot and solid-phase assays. In addition, purified Ro/SSA inhibited the anti-IgG activity of the anti-Ro/SSA sera from rabbit and man. Affinity purification of the IgG- and Ro/SSA-binding fractions of the rabbit anti-Ro/SSA demonstrated that both the anti-Ro/SSA and anti-IgG activities were concentrated in these fractions. These data show that Ro/SSA and IgG share epitopes that are bound by anti-Ro/SSA antibody. Inhibition experiments suggest that this antibody is found in most human anti-Ro/SSA autoimmune sera and that the epitope(s) are found in the F(ab')2 fragment of IgG.
摘要
风湿性疾病自身抗原Ro/SSA对兔宿主具有免疫原性。异源免疫兔血清在免疫印迹法和酶联免疫吸附测定中能与Ro/SSA颗粒结合。兔抗Ro/SSA血清和人抗Ro/SSA原型血清均能与IgG结合；此外，IgG可抑制兔和人抗Ro/SSA的活性。兔和人抗Ro/SSA血清的抗IgG活性通过蛋白质免疫印迹法和固相测定法与Ro/SSA结合。此外，纯化的Ro/SSA可抑制兔和人抗Ro/SSA血清的抗IgG活性。对兔抗Ro/SSA的IgG和Ro/SSA结合部分进行亲和纯化表明，抗Ro/SSA和抗IgG活性均集中在这些部分。这些数据表明，Ro/SSA和IgG具有抗Ro/SSA抗体所结合的共同表位。抑制实验表明，这种抗体存在于大多数人抗Ro/SSA自身免疫血清中，且该表位存在于IgG的F(ab')2片段中。
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