淋球菌H.8抗原的分离及初步生化特性分析
Isolation and preliminary biochemical characterization of the gonococcal H.8 antigen.
作者信息
Strittmatter W, Hitchcock P J
出版信息
J Exp Med. 1986 Dec 1;164(6):2038-48. doi: 10.1084/jem.164.6.2038.
Abstract
We have presented a method for the extraction and isolation of the gonococcal H.8 antigen. There was no evidence of contamination by other gonococcal proteins, phospholipids, or LPS. The purified H.8 antigen was subjected to preliminary analysis and appeared to be a proteolipid consisting of both protein and lipid components. The amino acid composition was unusual; the peptide portion of the antigen was an alanine and proline-rich molecule that lacked aromatic and sulfur-containing amino acids. The overall amino acid composition is hydrophobic. A lipid constituent was also identified; it was made up of at least two lipid components, which were unique to the H.8 molecule. The chemical nature of the association of the protein and lipid is presently unknown, but it is clearly a tenacious one.
摘要
我们提出了一种提取和分离淋球菌H.8抗原的方法。没有证据表明存在其他淋球菌蛋白、磷脂或脂多糖的污染。对纯化的H.8抗原进行了初步分析，它似乎是一种由蛋白质和脂质成分组成的蛋白脂质。其氨基酸组成不寻常；抗原的肽部分是一个富含丙氨酸和脯氨酸的分子，缺乏芳香族和含硫氨基酸。整体氨基酸组成具有疏水性。还鉴定出一种脂质成分；它由至少两种脂质成分组成，这些成分是H.8分子所特有的。目前尚不清楚蛋白质和脂质结合的化学性质，但显然是一种紧密的结合。
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