抗H.8单克隆抗体对脑膜炎球菌血症的明显抗性是由内毒素污染所致，而非特异性免疫保护。
Resistance to meningococcemia apparently conferred by anti-H.8 monoclonal antibody is due to contaminating endotoxin and not to specific immunoprotection.
作者信息
Woods J P, Black J R, Barritt D S, Connell T D, Cannon J G
出版信息
Infect Immun. 1987 Aug;55(8):1927-8. doi: 10.1128/iai.55.8.1927-1928.1987.
Abstract
We evaluated the ability of a monoclonal antibody directed against the common H.8 antigen of pathogenic Neisseria sp. to confer passive protection against meningococcal disease in mice. The apparent protection conferred by antibody purified from tissue culture supernatant was actually the result of endotoxin contamination of buffers and tissue culture media. Endotoxin-free anti-H.8 antibody was not protective. The possibility of endotoxin contamination should be considered when evaluating immunity conferred by passively administered antibody in animal models.
摘要
我们评估了一种针对致病性奈瑟菌属常见H.8抗原的单克隆抗体在小鼠中提供被动保护以抵御脑膜炎球菌病的能力。从组织培养上清液中纯化的抗体所赋予的明显保护实际上是缓冲液和组织培养基中内毒素污染的结果。无内毒素的抗H.8抗体没有保护作用。在评估动物模型中被动给予抗体所赋予的免疫力时，应考虑内毒素污染的可能性。
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