Division of Oral & Maxillary Surgery, Department of Stomatology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
Dental Laboratory Technology, Shu Zen College of Medicine and Management, Kaohsiung, Taiwan.
J Oral Pathol Med. 2019 Jul;48(6):468-476. doi: 10.1111/jop.12861. Epub 2019 Apr 29.
Type V collagen (COL5), in the functional heterotrimer [α1(V) α2(V)] isoform, participates in the malignancies of various cancers. However, its role in tongue squamous cell carcinoma (TSCC) remains unclear.
The expression levels of COL5A1 and COL5A2 polypeptide chains were examined using the tissue microarray from 245 TSCC patients with immunohistochemistry. Paired t test and Wilcoxon signed-rank test were performed for comparisons among the groups. Survival rates were estimated by using the Kaplan-Meier method and compared with log-rank tests. A Cox proportional hazards model was used to evaluate the impact of protein expression level on survival rate.
Expression level of COL5A1 was significantly increased in tumor tissues (P < 0.001) compared to that in corresponding adjacent normal tissues. High expression level of COL5A1 was associated with advanced pathological stage (III, IV, P = 0.015) and lymph node metastasis (P = 0.005) of TSCC patients. High expression level of COL5A1 was also correlated with poor disease-specific survival (DSS, P = 0.001) and disease-free survival (DFS, P = 0.003) in TSCC patients. However, high expression level of COL5A2 was correlated with better DFS in TSCC patients (P = 0.043). Moreover, co-expression level of high (COL5A1) /low (COL5A2) heterotrimer was correlated with worse DSS (P = 0.004) and DFS (P = 0.004).
COL5A1 is an unfavorable factor for tumorigenesis, clinicopathological outcomes, and prognosis, whereas COL5A2 is only a favorable factor for prognosis in TSCC. The co-expression of high (COL5A1)2/low (COL5A2) heterotrimer is a more potential unfavorable factor for prognosis in TSCC.
在功能异三聚体[α1(V)α2(V)]同种型中，V 型胶原（COL5）参与各种癌症的恶性转化。然而，其在舌鳞状细胞癌（TSCC）中的作用尚不清楚。
使用 245 例 TSCC 患者的组织微阵列，通过免疫组织化学方法检测 COL5A1 和 COL5A2 多肽链的表达水平。采用配对 t 检验和 Wilcoxon 符号秩检验对各组进行比较。采用 Kaplan-Meier 法估计生存率，并采用对数秩检验进行比较。采用 Cox 比例风险模型评估蛋白表达水平对生存率的影响。
COL5A1 的表达水平在肿瘤组织中明显高于相应的相邻正常组织（P<0.001）。COL5A1 高表达与 TSCC 患者的晚期病理分期（III、IV 期，P=0.015）和淋巴结转移（P=0.005）有关。COL5A1 高表达还与 TSCC 患者的疾病特异性生存率（DSS，P=0.001）和无病生存率（DFS，P=0.003）较差相关。然而，COL5A2 高表达与 TSCC 患者的DFS 较好相关（P=0.043）。此外，高（COL5A1）/低（COL5A2）异三聚体的共表达水平与较差的 DSS（P=0.004）和 DFS（P=0.004）相关。
COL5A1 是肿瘤发生、临床病理结果和预后的不利因素，而 COL5A2 仅是 TSCC 预后的有利因素。高（COL5A1）2/低（COL5A2）异三聚体的共表达是 TSCC 预后更具潜在不利因素。
