Fotino M
J Microsc. 1986 Sep;143(Pt 3):283-98. doi: 10.1111/j.1365-2818.1986.tb02785.x.
This article describes the operation and the characteristics of cryoHVEM imaging of biological specimens using a top-entry cryostage. The procedure for inserting frozen specimens into the microscope column is also presented. Whole mounts were observed under optimal imaging conditions by combining: fixation by fast freezing for structure preservation without exposure to chemicals, observation in the hydrated (frozen) state or in the dried state without exposure to the atmosphere after the initial fixation by freezing, and ultrastructural visualization with the key imaging factors of resolution, penetration and beam-induced damage at their best by high-voltage electron microscopy.
本文描述了使用顶部插入式低温样品台对生物标本进行低温高压电子显微镜（cryoHVEM）成像的操作及特点。还介绍了将冷冻标本插入显微镜柱体的步骤。通过以下方式相结合，在最佳成像条件下观察整装标本：通过快速冷冻进行固定以保存结构而不接触化学物质；在冷冻后的初始固定后，在水合（冷冻）状态或不暴露于大气的干燥状态下进行观察；以及通过高压电子显微镜，将分辨率、穿透率和束流诱导损伤等关键成像因素发挥到最佳水平，实现超微结构可视化。
