Fotino M, Giddings T H
J Ultrastruct Res. 1985 May;91(2):112-26. doi: 10.1016/0889-1605(85)90063-1.
A physical procedure for the visualization of cellular fine structures is described as an alternative to chemical preparative techniques. It consists of fixation by fast freezing followed by controlled etching in the cryostage of a million-volt transmission electron microscope. Whole mounts were thus observed under stable conditions with no use of chemical fixatives, solvents, or stains, with no exposure to the atmosphere, and with the improved penetration and resolution in thick specimens that characterize high-voltage electron microscopy. The preservation, contrast, and resolution exhibited by images of preparations obtained by this procedure are discussed.
本文描述了一种用于观察细胞精细结构的物理方法，作为化学制备技术的替代方法。该方法包括快速冷冻固定，然后在百万伏透射电子显微镜的低温台上进行可控蚀刻。通过这种方式，可以在稳定条件下观察整装标本，无需使用化学固定剂、溶剂或染色剂，无需暴露于大气中，并且在厚标本中具有高压电子显微镜特有的更好的穿透力和分辨率。本文还讨论了通过该方法获得的制剂图像所呈现的保存效果、对比度和分辨率。
