Department of Metallurgical Engineering and Materials, Federal University of Minas Gerais, School of Engineering, Belo Horizonte, MG, Brazil.
Biological Engineering Program, King Mongkut's University of Technology Thonburi, Thailand; Department of Materials, Imperial College London, South Kensington, London SW7 2AZ, UK.
J Colloid Interface Sci. 2019 Jul 1;547:382-392. doi: 10.1016/j.jcis.2019.04.016. Epub 2019 Apr 4.
Bioactive glass nanoparticles (BGNPs) are of great interest in tissue engineering as they possess high dissolution rate and capability of being internalized by cells, releasing their dissolution products with therapeutic benefits intracellularly. A modified Stöber process can be applied to obtain different BGNPs compositions containing therapeutic ions while maintaining controllable particle morphology, monodispersity and reduce agglomeration. Here, BGNPs containing Mn, an ion that has been shown to influence the osteoblast proliferation and bone mineralization, were evaluated. Particles with up to 142.3 ± 10.8 nm and spherical morphology were obtained after MnO incorporation in the SiO - CaO system. X-ray photoelectron spectroscopy (XPS) indicated the presence of Mn species and also a reduction in the number of bridging oxygen bonds due to the Ca and Mn. The Ca and Mn network modifier role on the silica network was also confirmed by magic-angle spinning Si solid-state nuclear magnetic resonance (MAS NMR). MTT evaluation showed no reduction in the mitochondrial metabolic activity of human mesenchymal stem cells exposed to the glass ionic products. Thus, evaluation showed that Mn could be incorporated into BGNPs by the modified Stöber method while maintaining their spherical morphology and features as a promising strategy for tissue regeneration.
生物活性玻璃纳米颗粒（BGNPs）在组织工程中具有很大的研究价值，因为它们具有高溶解率和被细胞内化的能力，能够将其溶解产物释放到细胞内，发挥治疗作用。改良的 Stöber 法可用于获得具有治疗作用的离子的不同 BGNPs 组成，同时保持可控的颗粒形态、单分散性和减少团聚。本研究中评估了含有 Mn 的 BGNPs，Mn 是一种被证明可以影响成骨细胞增殖和骨矿化的离子。在 SiO2-CaO 体系中掺入 MnO 后，可获得粒径高达 142.3±10.8nm 的球形颗粒。X 射线光电子能谱（XPS）表明存在 Mn 物种，并且由于 Ca 和 Mn 的存在，桥氧键的数量减少。通过魔角旋转硅固态核磁共振（MAS NMR）也证实了 Ca 和 Mn 对硅网络的网络修饰作用。MTT 评估表明，暴露于玻璃离子产物的人骨髓间充质干细胞的线粒体代谢活性没有降低。因此，评估结果表明，Mn 可以通过改良的 Stöber 法掺入 BGNPs 中，同时保持其球形形态和特征，这是一种很有前途的组织再生策略。
