Jiangsu Key Laboratory of Oral Diseases, Department of Prosthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, Jiangsu, China.
J Mater Sci Mater Med. 2020 Mar 5;31(3):29. doi: 10.1007/s10856-020-06368-8.
To validate the feasibility of two types of bioactive glass that contains spherical and radical spherical nano-sized particles in promoting bone repair, we hypothesize that radical spherical nano-sized particles have higher bone repair effectiveness than spherical one due to the physicochemical properties. We rigorously compared the physicochemical properties and bioactivities of these two types of bioactive glass. Specifically, we measured the size, surface morphology, concentration of ionic-dissolution products, bioactivity, and biological effects of two groups of bioactive glass on rat bone marrow mesenchymal stem cells (rBMSCs) and evaluate their effect on proliferation and osteogenic differentiation of rBMSCs in vitro. We observed that spherical nano-bioactive glass (SNBG) was spherical with smooth boundary, while the radial spherical nano-bioactive glass (RSNBG) had radial pore on the surface of particle boundary. When the two materials were immersed in simulated body fluid for 24 h, RSNBG produced more and denser hydroxyapatite carbonate than SNBG. The concentration of Ca and Si ions in RSNBG 24 h extract is higher than that of SNBG, while the concentration of P ions is lower. Proliferation, alkaline phosphatase (ALP) activity, intracellular Ca ion concentrations defined as the number of mineralized nodules produced, and the expression of osteogenic genes were significantly higher in rBMSCs co-cultured with 50 µg/mL RSNBG than SNBG. Overall, these results validated our hypothesis that RSNBG can provide better benefit than SNBG for inducing proliferation and osteogenic differentiation in rBMSCs, in turn suggested the feasibility of this RSNBG in further studies and utilization toward the ends of improved bone repair effectiveness.
为了验证含有球形和辐射状纳米级粒子的两种生物活性玻璃在促进骨修复方面的可行性，我们假设由于理化性质，辐射状纳米级粒子具有比球形更高的骨修复效果。我们严格比较了这两种类型的生物活性玻璃的理化性质和生物活性。具体来说，我们测量了两组生物活性玻璃的大小、表面形态、离子溶解产物浓度、生物活性以及对大鼠骨髓间充质干细胞(rBMSCs)的生物学效应，并评估了它们对 rBMSCs 体外增殖和成骨分化的影响。我们观察到，球形纳米生物活性玻璃(SNBG)呈球形且边界光滑，而辐射状纳米生物活性玻璃(RSNBG)在颗粒边界表面具有放射状孔。当两种材料在模拟体液中浸泡 24 小时时，RSNBG 比 SNBG 产生更多且更密集的碳酸羟基磷灰石。RSNBG 24 小时浸提液中 Ca 和 Si 离子的浓度高于 SNBG，而 P 离子的浓度较低。在与 50µg/mL RSNBG 共培养的 rBMSCs 中，增殖、碱性磷酸酶(ALP)活性、细胞内 Ca 离子浓度(定义为矿化结节的数量)和成骨基因的表达明显高于 SNBG。总的来说，这些结果验证了我们的假设，即 RSNBG 比 SNBG 更能促进 rBMSCs 的增殖和成骨分化，这反过来表明 RSNBG 在进一步的研究和利用中具有改善骨修复效果的可行性。
