University of Sadat City, Faculty of Veterinary Medicine, The Department of Bacteriology, Mycology, and Immunology, Menoufia, 32897, Egypt.
Infect Genet Evol. 2019 Jul;71:211-214. doi: 10.1016/j.meegid.2019.04.004. Epub 2019 Apr 8.
Mycobacterium bovis is a notorious infectious agent leading to serious economic losses for cattle farms worldwide. Analysis of the widely spreading genotypes is vital for tracing infections, understanding transmission dynamics, and controlling the cluster growth. This study aimed to evaluate the discrimination ability of 15 mycobacterial interspersed repetitive unit-variable number tandem repeats (MIRU-VNTR) loci and to assess the extremely efficient loci subset for molecular epidemiological investigations of M.bovis from farms in the Delta area of Egypt. The discriminating ability of MIRU-VNTR genotyping using 15 loci {2 exact tandem repeat (ETR) loci, 6 MIRU loci, 4 Mtub loci, and 3 Queen's University of Belfast (QUB) group loci)} were evaluated on 61 M.bovis isolates from cattle (Holstein Frisian) and buffaloes. The results indicate that there are 48 genotypes: 3 unique genotypes and 45 genotypes with shared similarities. Using the MIRU-VNTRplus database, M.bovis ID 7540/01 and ID 5346/02 were the nearest lineages to both groups. Six loci (MIRU10, QUB11b, QUB26, ETRA, Mtub30, and Mtub39) were highly discriminating, seven other loci (Mtub21, MIRU26, QUB4156, MIRU04 (ETRD), MIRU16, MIRU 40, and ETRC) gave moderate discriminatory power, and the last two loci (Mtub04 and MIRU31) were poorly discriminative. MIRU-VNTR typing generally proved efficacy and high discriminatory power, with a collective allele's diversification of 0.9641. Both the six highly discriminating (DI = 0.9492) and the seven moderately discriminating loci (DI = 0.9269) evidenced to be suitable for M.bovis first-step initial genotyping from cattle herds in Egypt. MIRU-VNTR is rapid and effective in the genotyping of M.bovis from cattle and buffaloes in Egypt.
牛分枝杆菌是一种臭名昭著的传染性病原体，给全世界的奶牛场造成了严重的经济损失。分析广泛传播的基因型对于追踪感染、了解传播动态和控制集群增长至关重要。本研究旨在评估 15 个分枝杆菌间隔重复单元-可变数串联重复（MIRU-VNTR）基因座的区分能力，并评估用于评估来自埃及三角洲地区农场的牛分枝杆菌的分子流行病学研究的极其有效的基因座子集。使用 15 个基因座（2 个精确串联重复（ETR）基因座、6 个 MIRU 基因座、4 个 Mtub 基因座和 3 个贝尔法斯特女王大学（QUB）组基因座）对 61 株来自牛（荷斯坦弗里森）和水牛的牛分枝杆菌分离株进行 MIRU-VNTR 基因分型的区分能力进行了评估。结果表明，有 48 种基因型：3 种独特基因型和 45 种具有相似性的基因型。使用 MIRU-VNTRplus 数据库，ID 7540/01 和 ID 5346/02 与两组最接近。6 个基因座（MIRU10、QUB11b、QUB26、ETRA、Mtub30 和 Mtub39）具有高度的区分能力，另外 7 个基因座（Mtub21、MIRU26、QUB4156、MIRU04（ETRD）、MIRU16、MIRU 40 和 ETRC）具有中等区分能力，最后两个基因座（Mtub04 和 MIRU31）区分能力较差。MIRU-VNTR 分型通常证明具有功效和高度区分能力，其共同等位基因的多样化程度为 0.9641。6 个高度区分的基因座（DI=0.9492）和 7 个中度区分的基因座（DI=0.9269）都证明适用于埃及牛群中牛分枝杆菌的初步初始基因分型。MIRU-VNTR 快速有效，可用于对埃及牛和水牛中的牛分枝杆菌进行基因分型。
