Yang Li, Wang Chunyu, Wang Haijun, Meng Qingfeng, Wang Quankai
College of Chinese Medicinal Materials, Jilin Agricultural University, Xincheng Street No.2888, 130118, Changchun, China.
Jilin Entry-Exit Inspection and Quadrant Bureau, Puyang Street No.1301, Changchun, 130062, China.
BMC Vet Res. 2015 Apr 11;11:93. doi: 10.1186/s12917-015-0402-0.
Bovine tuberculosis has led to serious economic losses for Sika Deer producers in China. Strategies for controlling the spread of Mycobacterium bovis are often hampered by a lack of epidemiological data. Specifically, tracing infections requires the ability to trace back infections, which, in turn, requires the ability to determine isolates with a common source. This study was planned to assess the discriminatory power of each mycobacterial interspersed repetitive unit (MIRU)-variable number tandem repeats (VNTR) locus and evaluate the most appropriate combination of MIRU-VNTR loci for molecular epidemiological studies on Sika Deer in China.
The discriminatory power of MIRU-VNTR typing based on 22 known loci (12 MIRUs, 2 ETRs, 4 QUBs, and 4 Mtubs) were assessed in 96 Mycobacterium bovis strains collected sequentially from Sika Deer at a slaughterhouse in northeastern China. We defined four loci (MIRU4, ETRA, QUB11b, and Mtub4) as highly discriminative, eight loci (MIRU2, MIRU23, MIRU27, MIRU31, MIRU39, MIRU40, QUB26, and Mtub21) as moderately discriminative, and three loci (MIRU16, Mtub30, and Mtub34) as poorly discriminative. The final locus showed no polymorphism between strains. MIRU-VNTR typing as a whole was highly discriminative, with an overall allelic diversity of 0.897. Of the loci tested, the four highly discriminative loci and eight moderately discriminative loci proved to be most appropriate for first line typing of M. bovis from Sika Deer, with the same resolving ability as all 22 loci (H = 0.897).
MIRU-VNTR typing is quick and effective for typing bovine tuberculosis isolates from Sika Deer in China.
牛结核病给中国梅花鹿养殖户造成了严重的经济损失。牛分枝杆菌传播控制策略常因缺乏流行病学数据而受阻。具体而言，追踪感染需要具备追溯感染源的能力，而这又需要能够确定具有共同来源的分离株。本研究旨在评估每个分枝杆菌散布重复单元（MIRU）-可变数目串联重复序列（VNTR）位点的鉴别力，并评估MIRU-VNTR位点的最合适组合，用于中国梅花鹿的分子流行病学研究。
在中国东北一家屠宰场从梅花鹿身上依次采集的96株牛分枝杆菌菌株中，评估了基于22个已知位点（12个MIRU、2个ETR、4个QUB和4个Mtub）的MIRU-VNTR分型的鉴别力。我们将四个位点（MIRU4、ETRA、QUB11b和Mtub4）定义为高鉴别力位点，八个位点（MIRU2、MIRU23、MIRU27、MIRU31、MIRU39、MIRU40、QUB26和Mtub21）定义为中等鉴别力位点，三个位点（MIRU16、Mtub30和Mtub34）定义为低鉴别力位点。最后一个位点在菌株之间未显示多态性。总体而言，MIRU-VNTR分型具有高度鉴别力，总体等位基因多样性为0.897。在所测试的位点中，四个高鉴别力位点和八个中等鉴别力位点被证明最适合对来自梅花鹿的牛分枝杆菌进行一线分型，其分辨能力与所有22个位点相同（H = 0.897）。
MIRU-VNTR分型对于中国梅花鹿牛结核分离株的分型快速有效。
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