Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand.
Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, 65000, Thailand.
Phytochemistry. 2019 Jul;163:11-22. doi: 10.1016/j.phytochem.2019.03.017. Epub 2019 Apr 8.
Plant cells have a variety of defense mechanisms to alleviate the deleterious effects of oxidative stress. The present work elucidated a schematic diagram of the proposed pathway of peanut hairy root tissue treated with different elicitors; paraquat (PQ), methyl jasmonate (MeJA), and cyclodextrin (CD). The different elicitation approaches could provoke intrinsic stress in plant cells and might activate a distinct response pathway, allowing plants to overcome the deleterious effects of oxidative stress. Among all strategies, hairy root culture pretreated with PQ followed by application of MeJA plus CD showed an extensive induction of antioxidant defense mechanisms. The expression of the antioxidant enzyme genes and stilbene-synthesized enzyme genes were up-regulated in accordance with the dramatic increase in the production of stilbene compounds. The non-enzymatic antioxidant substances exhibited a highly enhanced capability. The pathogenesis-related protein (PR) genes were also highly up-regulated. In summary, we demonstrated that the interplay among MeJA plus CD and PQ may activate a complex signaling network to regulate plant defense mechanisms involving the up-regulation of detoxifying enzymes, induction of free-radical scavengers and overexpression of genes associated with plant defense pathways.
植物细胞具有多种防御机制来减轻氧化应激的有害影响。本研究阐明了不同诱导剂（百草枯（PQ）、茉莉酸甲酯（MeJA）和环糊精（CD））处理花生毛状根组织的拟议途径的示意图。不同的诱导方法可能会引起植物细胞的内在应激，并可能激活不同的反应途径，使植物能够克服氧化应激的有害影响。在所有策略中，用 PQ 预处理毛状根，然后再用 MeJA 和 CD 处理，表现出抗氧化防御机制的广泛诱导。抗氧化酶基因和芪类合成酶基因的表达随着芪类化合物产量的急剧增加而上调。非酶抗氧化物质的能力也得到了极大的提高。病程相关蛋白（PR）基因也高度上调。总之，我们证明了 MeJA 加 CD 和 PQ 之间的相互作用可能会激活一个复杂的信号网络，调节植物防御机制，包括解毒酶的上调、自由基清除剂的诱导以及与植物防御途径相关基因的过表达。
