Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand.
Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, 65000, Thailand.
J Plant Physiol. 2020 Nov;254:153278. doi: 10.1016/j.jplph.2020.153278. Epub 2020 Sep 17.
Plants counteract Cd toxicity by activating cellular stress responses. The simultaneous exogenous application of methyl jasmonate (MeJA) and methyl-β-cyclodextrin (CD) before Cd exposure improved the response of Arachis hypogaea hairy root culture to the unfavourable effects of Cd toxicity. At 24 h after elicitation, genes that encode key enzymes in the phenylpropanoid biosynthesis pathway (i.e., PAL and RS3) were up-regulated to 3.2- and 5.4-fold changes respectively, thereby inducing stilbene production. The up-regulation of genes that encode transcription factors (i.e., ERF1 and ERF6) significantly increased the expression of several genes (PR4A, PR5, PR10, and chitinase) that encode the pathogenesis-related (PR) proteins to 25.8-, 45-, 5- and 12.6-fold changes, respectively. The more dramatic up-regulation of PR protein-encoding genes demonstrated the significant role of defence proteins in plant protective mechanisms. The prolonged (i.e., 72-h) treatment with MeJA + CD_Cd triggered adaptive responses by substantially increasing the levels of antioxidants, stilbenes, and other phenolic substances. These findings suggest that the interaction between signalling elicitors (MeJA and CD) and Cd modulates a complex signalling network for plant defence system.
植物通过激活细胞应激反应来抵抗 Cd 毒性。在 Cd 暴露前同时施加茉莉酸甲酯（MeJA）和甲基-β-环糊精（CD），可以提高花生毛状根培养物对 Cd 毒性不利影响的反应。在诱导 24 小时后，编码苯丙烷生物合成途径中关键酶的基因（即 PAL 和 RS3）分别上调至 3.2 倍和 5.4 倍，从而诱导芪类物质的产生。转录因子（即 ERF1 和 ERF6）编码基因的上调，显著增加了几类基因（PR4A、PR5、PR10 和几丁质酶）的表达，这些基因编码的是病程相关（PR）蛋白，分别上调至 25.8 倍、45 倍、5 倍和 12.6 倍。PR 蛋白编码基因的上调程度更大，表明防御蛋白在植物保护机制中起着重要作用。用 MeJA+CD_Cd 进行延长（即 72 小时）处理，通过显著增加抗氧化剂、芪类物质和其他酚类物质的水平，引发了适应性反应。这些发现表明，信号激发剂（MeJA 和 CD）与 Cd 之间的相互作用调节了植物防御系统的复杂信号网络。
