Bunker J C, James A M
Microbios. 1986;47(192-193):177-88.
A study has been made of the effects of media and oxygen tension on the power-time (p-t) traces exhibited by cells of Klebsiella aerogenes, Staphylococcus aureus and Pseudomonas aeruginosa. The p-t traces were more complex in shape when the cells were grown in rich nutrient medium than when grown in chemically-defined glucose-limited medium. There was less waste heat and more biomass was produced during growth in nutrient broth. The oxygen tension had a profound effect on the shape of the p-t traces, and two strains of S. aureus, which differed in antibiotic susceptibility, exhibited different traces at the same oxygen tension. The thermal yield of the resistant strain was less than that of the sensitive strain. The choice of medium and the level of aeration, rather than the organism, determined the shape of the p-t trace. This has serious consequences for the proposed use of microcalorimetry as a rapid method for bacterial identification.
一项针对培养基和氧张力对产气克雷伯菌、金黄色葡萄球菌和铜绿假单胞菌细胞所呈现的功率-时间（p-t）曲线的影响进行了研究。当细胞在丰富营养培养基中生长时，p-t曲线的形状比在化学限定的葡萄糖限制培养基中生长时更为复杂。在营养肉汤中生长期间，废热较少且产生的生物量更多。氧张力对p-t曲线的形状有深远影响，并且两株对抗生素敏感性不同的金黄色葡萄球菌在相同氧张力下呈现出不同的曲线。耐药菌株的热产率低于敏感菌株。培养基的选择和通气水平而非生物体决定了p-t曲线的形状。这对于将微量热法作为细菌鉴定的快速方法的提议应用具有严重影响。
