Egger S F, Buxbaum A, Georgopoulos M, Scholda C, Vecsei V P, Huber-Spitzy V, Georgopoulos A
University Eye Clinic, University of Vienna, Austria.
Exp Eye Res. 1997 Dec;65(6):791-5. doi: 10.1006/exer.1997.0385.
The aim of this study was to investigate the capacity of human vitreous to support bacterial growth and to show differences in the growth kinetics of gram-positive and gram-negative bacteria. Vitreous gel of 70 keratoplasty donor eyes was sampled under sterile conditions, screened microscopically for cellular components and tested for sterility and levels of antibiotic drugs by bio-assay. The samples were inoculated with clinical isolates of Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus viridans and Streptococcus pyogenes. As control each strain was added both to 0.9% sodium chloride solution and to Mueller-Hinton broth. In order to determine bacterial growth the number of colony forming units was determined 4, 6, 24, 48 and 72 hr after inoculation by viable count. Vitreous gel did not support bacterial growth; the tested strains could not be recovered after 48 hr. Similar results could be obtained with sodium chloride; whereas in Mueller Hinton broth the strains showed normal pattern of growth. It seems that vitreous humor has inherent antibacterial capacity in vitro, although the responsible factors remain unknown.
本研究的目的是调查人玻璃体支持细菌生长的能力，并显示革兰氏阳性菌和革兰氏阴性菌生长动力学的差异。在无菌条件下采集70只角膜移植供体眼的玻璃体凝胶，通过显微镜检查筛选细胞成分，并通过生物测定法检测无菌性和抗生素药物水平。将样品接种铜绿假单胞菌、肺炎克雷伯菌、金黄色葡萄球菌、表皮葡萄球菌、草绿色链球菌和化脓性链球菌的临床分离株。作为对照，将每种菌株分别添加到0.9%氯化钠溶液和穆勒-欣顿肉汤中。为了确定细菌生长情况，接种后4、6、24、48和72小时通过活菌计数确定菌落形成单位的数量。玻璃体凝胶不支持细菌生长；48小时后无法回收测试菌株。氯化钠也可得到类似结果；而在穆勒-欣顿肉汤中，菌株显示出正常的生长模式。玻璃体似乎在体外具有内在的抗菌能力，尽管其相关因素尚不清楚。
