Bygd Hannah C, Bratlie Kaitlin M
Department of Materials Science & Engineering, Iowa State University, Ames, IA 50011, USA.
Department of Chemical & Biological Engineering, Iowa State University, Ames, IA 50011, USA.
Polymers (Basel). 2016 Dec 6;8(12):422. doi: 10.3390/polym8120422.
Understanding macrophage responses to biomaterials is crucial to the success of implanted medical devices, tissue engineering scaffolds, and drug delivery vehicles. Cellular responses to materials may depend synergistically on multiple surface chemistries, due to the polyvalent nature of cell⁻ligand interactions. Previous work in our lab found that different surface functionalities of chemically modified alginate could sway macrophage phenotype toward either the pro-inflammatory or pro-angiogenic phenotype. Using these findings, this research aims to understand the relationship between combined material surface chemistries and macrophage phenotype. Tumor necrosis factor-α (TNF-α) secretion, nitrite production, and arginase activity were measured and used to determine the ability of the materials to alter macrophage phenotype. Cooperative relationships between pairwise modifications of alginate were determined by calculating synergy values for the aforementioned molecules. Several materials appeared to improve M1 to M2 macrophage reprogramming capabilities, giving valuable insight into the complexity of surface chemistries needed for optimal incorporation and survival of implanted biomaterials.
了解巨噬细胞对生物材料的反应对于植入式医疗设备、组织工程支架和药物递送载体的成功至关重要。由于细胞与配体相互作用的多价性质，细胞对材料的反应可能协同依赖于多种表面化学性质。我们实验室之前的工作发现，化学修饰藻酸盐的不同表面功能可以使巨噬细胞表型向促炎或促血管生成表型转变。利用这些发现，本研究旨在了解材料表面化学组合与巨噬细胞表型之间的关系。测量肿瘤坏死因子-α（TNF-α）分泌、亚硝酸盐产生和精氨酸酶活性，并用于确定材料改变巨噬细胞表型的能力。通过计算上述分子的协同值来确定藻酸盐成对修饰之间的协同关系。几种材料似乎提高了M1到M2巨噬细胞重编程能力，为植入生物材料的最佳整合和存活所需的表面化学复杂性提供了有价值的见解。
