Laboratory of Immunopathology of infectious diseases, Department of Immunology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Rua 235, Goiania, 74605-050 Goiás, Brazil.
Laboratory of Neuropharmacology, Department of Physiological Sciences, Institute of Biological Sciences, University of Brasília, 70910-900 Brasilia, Brazil.
Toxins (Basel). 2019 Apr 10;11(4):216. doi: 10.3390/toxins11040216.
Intravascular stent infection is a rare complication with a high morbidity and high mortality; bacteria from the hospital environment form biofilms and are often multidrug-resistant (MDR). Antimicrobial peptides (AMPs) have been considered as alternatives to bacterial infection treatment. We analyzed the formation of the bacterial biofilm on the vascular stents and also tested the inhibition of this biofilm by AMPs to be used as treatment or coating. Antimicrobial activity and antibiofilm were tested with wasp (Agelaia-MPI, Polybia-MPII, Polydim-I) and scorpion (Con10 and NDBP5.8) AMPs against clinical strains. formed a biofilm on the vascular stent. Agelaia-MPI and Polybia-MPII inhibited biofilm formation with bacterial cell wall degradation. Coating biofilms with polyethylene glycol (PEG 400) and Agelaia-MPI reduced 90% of adhesion on stents. The wasp AMPs Agelaia-MPI and Polybia-MPII had better action against MDR adherence and biofilm formation on vascular stents, preventing its formation and treating mature biofilm when compared to the other tested peptides.
血管内支架感染是一种罕见的并发症，发病率和死亡率都很高；医院环境中的细菌会形成生物膜，且通常具有多重耐药性（MDR）。抗菌肽（AMPs）被认为是治疗细菌感染的替代方法。我们分析了血管支架上细菌生物膜的形成，还测试了 AMPs 对这种生物膜的抑制作用，以用作治疗或涂层。我们使用黄蜂（Agelaia-MPI、Polybia-MPII、Polydim-I）和蝎子（Con10 和 NDBP5.8）AMPs 对临床分离株进行了抗菌活性和抗生物膜测试。临床分离株在血管支架上形成了生物膜。Agelaia-MPI 和 Polybia-MPII 通过降解细菌细胞壁来抑制生物膜形成。用聚乙二醇（PEG 400）和 Agelaia-MPI 涂层生物膜可减少 90%的 黏附在支架上的细菌。与其他测试肽相比，黄蜂 AMPs Agelaia-MPI 和 Polybia-MPII 对 MDR 血管支架上的 黏附和生物膜形成具有更好的作用，可预防其形成并治疗成熟的生物膜。
