Stem Cells Therapies in Neurodegenerative Diseases Lab, Research Center "Principe Felipe", Valencia 46012, Spain.
Department of Microbiology and Ecology, University of Valencia, Valencia 46100, Spain.
Toxins (Basel). 2019 Apr 10;11(4):217. doi: 10.3390/toxins11040217.
Ochratoxin A (OTA) is a mycotoxin produced by different and species, and it is considered a common contaminant in food and animal feed worldwide. On the other hand, human embryonic stem cells (hESCs) have been suggested as a valuable model for evaluating drug embryotoxicity. In this study, we have evaluated potentially toxic effects of OTA in hESCs. By using in vitro culture techniques, specific cellular markers, and molecular biology procedures, we found that OTA produces mild cytotoxic effects in hESCs by inhibiting cell attachment, survival, and proliferation in a dose-dependent manner. Thus, we suggest that hESCs provide a valuable human and cellular model for toxicological studies regarding preimplantation stage of human fetal development.
赭曲霉毒素 A（OTA）是由不同的 和 真菌产生的一种真菌毒素，被认为是全世界食品和动物饲料中常见的污染物。另一方面，人类胚胎干细胞（hESCs）已被提议作为评估药物胚胎毒性的有价值的模型。在这项研究中，我们评估了 OTA 在 hESCs 中的潜在毒性作用。通过使用体外培养技术、特定的细胞标记物和分子生物学程序，我们发现 OTA 通过剂量依赖性方式抑制细胞附着、存活和增殖，从而对 hESCs 产生轻微的细胞毒性作用。因此，我们认为 hESCs 为关于人类胎儿发育植入前阶段的毒理学研究提供了有价值的人类和细胞模型。
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