Sun Xuejing, Liu Enxue, Iqbal Adeela, Wang Taozhi, Wang Xindong, Haseeb Abdul, Ahmed Nisar, Yang Ping, Chen Qiusheng
Ministry of Education Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Wei gang No.1, Nanjing, 210095, Jiangsu Province, China.
BMC Vet Res. 2019 Apr 11;15(1):112. doi: 10.1186/s12917-019-1860-6.
Duck Tembusu virus (DTMUV) is a novel member of Flavivirus. The isolated and purified DTMUV strain XZ-2012 was used as a strain model, to intramuscularly inject the six-month egg-laying shelducks with the infective dose of 10TCID. The dynamic distribution of the virus in spleen at different time post-infection (pi) was studied using RT-PCR, RT-qPCR, ELISA, immunofluorescence and transmission electron microscopy (TEM).
The results showed that the virus occurred in the spleen after 2 hpi and lasted up to 18 dpi. The registered viral load increased from 2 hpi to 3 dpi, and then it diminished from 6 dpi to 18 dpi with a slight rise at 12 dpi. From 2 hpi to 6 dpi the DTMUV particles were mostly distributed in the periellipsoidal lymphatic sheath (PELS) of spleen white pulp, few being found in the sheathed capillary. From 9 dpi to 18 dpi, the DTMUV particles were migrating into periarterial lymphatic sheaths (PALS) around the central artery through the red pulp. Under TEM, the virus particles could be observed mostly in lymphocytes and macrophages.
It was suggested that DTMUV invaded lymphocytes and macrophages of the spleen at 2 hpi and replicated significantly from 1 dpi to 3 dpi, being eliminated from 9 dpi to 18 dpi. This is the first study on the dynamic distribution of DTMUV from invasion to elimination in duck spleen conducted by molecular and morphological methods. It could provide theoretical basis for the occurrence, development and detoxification of the virus in the organs of the immune system.
鸭坦布苏病毒（DTMUV）是黄病毒属的一个新成员。以分离纯化的DTMUV XZ - 2012株为毒株模型，对6月龄产蛋麻鸭进行肌肉注射，感染剂量为10TCID。采用RT - PCR、RT - qPCR、ELISA、免疫荧光和透射电子显微镜（TEM）研究病毒在感染后不同时间点在脾脏中的动态分布。
结果显示，病毒在感染后2小时出现在脾脏中，并持续到感染后18天。记录的病毒载量从感染后2小时到3天增加，然后从感染后6天到18天减少，在12天时略有上升。从感染后2小时到6天，DTMUV颗粒主要分布在脾白髓的椭球周围淋巴鞘（PELS）中，在被膜毛细血管中很少发现。从感染后9天到18天，DTMUV颗粒通过红髓迁移到中央动脉周围的动脉周围淋巴鞘（PALS）中。在透射电子显微镜下，病毒颗粒主要在淋巴细胞和巨噬细胞中观察到。
提示DTMUV在感染后2小时侵入脾脏的淋巴细胞和巨噬细胞，并在感染后1天到3天大量复制，在感染后9天到18天被清除。这是首次通过分子和形态学方法对DTMUV在鸭脾脏中从侵入到清除的动态分布进行的研究。可为病毒在免疫系统器官中的发生、发展和排毒提供理论依据。
