Department of Animal Sciences, Food and Halieutic Resources, National Institute of Agronomy of Tunisia, Tunis, Tunisia; Laboratory of Materials, Molecules and Application, Preparatory Institute for Scientific and Technical Studies, Tunis, Tunisia.
Laboratory of Bioagressors and Integrated Protection in Agriculture, National Institute of Agronomy of Tunisia, Tunis, Tunisia.
J Therm Biol. 2019 Apr;81:73-81. doi: 10.1016/j.jtherbio.2019.02.018. Epub 2019 Feb 27.
This study aims to investigate the thermoprotective properties of Opuntia ficus-indica f. inermis. Extracts were prepared from cladodes (CE) and mesocarps (ME), then subjected to a spectrophotometric and LC-MS analyses. Lymphocytes were isolated from peripheral blood of non-stressed sheep, supplemented with CE, ME, betanin or α-tocopherol, and subjected to two thermal treatments: 40 and 41 °C, for 6 h. Viable lymphocytes and HO production were evaluated. The antioxidant activity of ME was 3.43 folds higher than CE. The LC-MS analysis of CE and ME allowed identifying 11 phenolic acids, 2 flavanones, 6 flavones, 3 flavonols and 1 betanin type betacyanin. Lymphocytes mortality increased linearly as function of the severity and the duration of heat stress. This mortality was correlated with HO production. At 41 °C, only ME allowed maintaining lymphocytes viability. Moreover, ME was more efficient than CE in reducing HO production. This thermoprotection was ensured by betaxanthin and betacyanin pigments. Interestingly, betanin was more efficient than α-tocopherol in preventing hyperthermia-induced lymphocytes' mortality. We report here for the first time the thermoprotective properties of cladodes and mesocarps of Opuntia ficus-indica f. inermis. Betanin was able to maintain lymphocyte viability through reducing HO production, and therefore the oxidative-induced heat stress.
本研究旨在探讨仙人掌果实的耐热特性。从刺梨（CE）和中果皮（ME）中提取提取物，然后进行分光光度法和 LC-MS 分析。从非应激绵羊的外周血中分离淋巴细胞，用 CE、ME、甜菜碱或 α-生育酚补充，并进行两种热处理：40 和 41°C，持续 6 小时。评估活淋巴细胞和 HO 产生。ME 的抗氧化活性比 CE 高 3.43 倍。CE 和 ME 的 LC-MS 分析鉴定出 11 种酚酸、2 种黄烷酮、6 种黄酮、3 种黄酮醇和 1 种甜菜碱型甜菜红素。淋巴细胞死亡率随热应激的严重程度和持续时间呈线性增加。这种死亡率与 HO 的产生有关。在 41°C 时，只有 ME 能够维持淋巴细胞的活力。此外，ME 在降低 HO 产生方面比 CE 更有效。这种耐热性是由甜菜黄素和甜菜红素类色素提供的。有趣的是，与 α-生育酚相比，甜菜碱更能防止高温诱导的淋巴细胞死亡。我们首次报道了仙人掌果实的耐热特性。通过降低 HO 产生，甜菜碱能够维持淋巴细胞的活力，从而减轻氧化诱导的热应激。
