Mata A, Ferreira J P, Semedo C, Serra T, Duarte C M M, Bronze M R
Faculdade de Farmácia da Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-019 Lisboa, Portugal.
Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2780-901 Oeiras, Portugal; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal.
Food Chem. 2016 Nov 1;210:558-65. doi: 10.1016/j.foodchem.2016.04.033. Epub 2016 Apr 13.
Opuntia spp. fruits are considered as health promoting foods due to the diversity of bioactive molecules found in these fruits. The composition in organic acids, flavonols and betalains in the Opuntia ficus-indica juice from a region of Portugal was accomplished for the first time by liquid chromatography and tandem mass spectrometry using an electrospray ionization source operating in negative and positive mode. The methodology used allowed the detection of 44 compounds, from which 32 were identified. Isorhamnetin derivatives were the dominant flavonol glycosides. A total of 9 betalains including 6 betaxanthins and 3 betacyanin were also detected in the fruit juice samples and indicaxanthin, betanin and isobetanin were the major pigments. Phenolic acid and phenylpyruvic acid derivatives were also identified. To our knowledge, it is the first time derivative compounds from piscidic acid, phenolic compounds and betalains are characterized in cactus pear juice using a single LC-DAD-ESI-MS/MS method.
由于仙人掌属果实中发现的生物活性分子具有多样性，仙人掌属果实被认为是促进健康的食物。首次采用液相色谱和串联质谱法，使用在正负模式下运行的电喷雾电离源，对葡萄牙某地区的仙人掌果汁中的有机酸、黄酮醇和甜菜色素进行了成分分析。所采用的方法能够检测出44种化合物，其中32种已被鉴定。异鼠李素衍生物是主要的黄酮醇苷。在果汁样品中还共检测到9种甜菜色素，包括6种甜菜黄素和3种甜菜红素，其中仙人掌黄质、甜菜红苷和异甜菜红苷是主要色素。还鉴定出了酚酸和苯丙酮酸衍生物。据我们所知，这是首次使用单一的液相色谱-二极管阵列检测器-电喷雾串联质谱法对仙人掌果汁中的鱼藤酸衍生物、酚类化合物和甜菜色素进行表征。
