Datta Sudeshna, Sinha B K, Bhattacharjee Soumen, Seal Tapan
Industrial Section, Indian Museum, Botanical Survey of India, Kolkata, India.
Botanical Survey of India, CGO Complex, DF Block, Sector-I, Salt Lake City, Kolkata, India.
Heliyon. 2019 Mar 28;5(3):e01431. doi: 10.1016/j.heliyon.2019.e01431. eCollection 2019 Mar.
The present study aimed to investigate the nutritional properties and antioxidant activities of six underutilized wild edible plants . , and that are commonly consumed as food in the India The antioxidant properties of the plants were evaluated by using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, ABTS radical scavenging ability, reducing power capacity, metal chelating activity, lipid peroxidation assay, estimation of total phenolic content and flavonoids content in different solvent extraction system like benzene, chloroform, methanol and 70% aqueous (aq.) ethanol. The quantitation of phenolic acids and flavonoids and water soluble vitamins in these plants were carried out by HPLC using Acclaim C 18 column (5 μm particle size, 250 × 4.6 mm), Dionex Ultimate 3000 liquid chromatograph and detection was carried out in photo diode array (PDA) detector. The results of investigation showed that these plants are rich sources of protein, carbohydrate minerals and vitamins, especially the B group of vitamins that can contribute immensely to nutrition, food security, and health and therapeutic benefits. The different levels of antioxidant activities were found in the solvent systems used. The HPLC analysis also showed the presence of phenolic acids and flavonoids in various amounts in these plants which could be utilized as natural antioxidant.
本研究旨在调查六种未充分利用的野生可食用植物的营养特性和抗氧化活性。这六种植物分别是[此处原文缺失植物名称]，在印度它们通常被作为食物食用。通过1,1-二苯基-2-苦基肼（DPPH）自由基清除活性、ABTS自由基清除能力、还原能力、金属螯合活性、脂质过氧化测定、在苯、氯仿、甲醇和70%乙醇水溶液等不同溶剂萃取体系中总酚含量和黄酮类化合物含量的测定来评估这些植物的抗氧化特性。使用Acclaim C 18柱（粒径5μm，250×4.6mm）、戴安Ultimate 3000液相色谱仪，通过高效液相色谱法对这些植物中的酚酸、黄酮类化合物和水溶性维生素进行定量，并在光电二极管阵列（PDA）检测器中进行检测。调查结果表明，这些植物富含蛋白质、碳水化合物、矿物质和维生素，尤其是B族维生素，它们对营养、粮食安全以及健康和治疗益处有巨大贡献。在所使用的溶剂体系中发现了不同水平的抗氧化活性。高效液相色谱分析还表明，这些植物中存在不同含量的酚酸和黄酮类化合物，它们可作为天然抗氧化剂。
