Department of Botany, School of Life Sciences, Bharathiar University, Coimbatore, 641046 TN India.
Department of Environmental Sciences, School of Life Sciences, Bharathiar University, Coimbatore, 641046 TN India.
J Food Sci Technol. 2013 Aug;50(4):687-95. doi: 10.1007/s13197-011-0389-x. Epub 2011 May 5.
In the present study, antioxidant activities of the phenolic extracts from H. isora fruits and C. pentandra seeds were investigated by employing established in vitro systems, which included reducing power, OH(●), DPPH(●), ABTS(●+), linoleic acid emulsion, metal chelation and antihemolytic activity. The extracts of C. pentandra contained relatively higher levels of total phenolics and flavonoids than those of H. isora. All the extracts showed dose dependent reducing power activity and moreover, they were well correlated with the total phenolic substances. A similar dose dependant trend has also been observed for hydroxyl radical scavenging activity and DPPH(●) radical scavenging activity. Further, addition of 250 μg of extracts to the reaction mixture produced 41.3-54.6% peroxidation inhibiting activity during 60 h of incubation. The potential of multiple antioxidant activity of samples can be further evidenced by inhibition of reactive oxygen mediated erythrocyte cell lysis and metal ion chelating activity.
在本研究中，通过使用已建立的体外系统，研究了 H. isora 果实和 C. pentandra 种子酚类提取物的抗氧化活性，其中包括还原能力、OH(●)、DPPH(●)、ABTS(●+)、亚油酸乳液、金属螯合和抗溶血活性。C. pentandra 的提取物比 H. isora 含有相对更高水平的总酚类物质和类黄酮。所有提取物均表现出剂量依赖性的还原能力活性，并且与总酚类物质具有良好的相关性。羟基自由基清除活性和 DPPH(●)自由基清除活性也呈现出类似的剂量依赖性趋势。此外，在 60 小时的孵育过程中，向反应混合物中添加 250μg 的提取物可产生 41.3-54.6%的过氧化抑制活性。通过抑制活性氧介导的红细胞细胞裂解和金属离子螯合活性，可以进一步证明样品的多种抗氧化活性的潜力。
