Ghadage Dhanaji M, Kshirsagar Parthraj R, Pai Sandeep R, Chavan Jaykumar J
Department of Botany, Yashavantrao Chavan Institute of Science, Satara 415001, India.
Department of Biotechnology, Shivaji University, Kolhapur 416004, India.
Biochem Biophys Rep. 2017 Aug 24;12:79-90. doi: 10.1016/j.bbrep.2017.08.012. eCollection 2017 Dec.
The study aimed to evaluate extraction efficiency, detection and quantification of phytochemicals, minerals and antioxidative capacity of different parts of L. Continuous shaking extraction, steam bath assisted extraction, ultrasonic extraction and microwave assisted extraction with varied time intervals were employed for extraction of phenolics, flavonoids, and antioxidants. Preliminary screening revealed the presence of wide array of metabolites along with carbohydrates and starch. Steam bath assisted extraction for 10 min exposure was found most suitable for extraction phenolics (46.02 ± 2.30 mg of gallic acid equivalent per gram of dry weight and 48.57 ± 2.42 mg of tannic acid equivalent per gram of dry weight) and flavonoids (35.26 ± 1.61 mg of quercetin equivalent per gram of dry weight and 51.60 ± 2.58 mg of ellagic acid equivalent per gram of dry weight). In support, reverse phase-high performance liquid chromatography- diode array detector confirmed the presence of seven pharmaceutically important phenolic acids. Antioxidant capacity was measured by 1, 1- diphenyl-1-picryl hydrazyl (DPPH), ferric reducing antioxidant power (FRAP), 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) scavenging (ABTS) and N, N-dimethyl-p-phenylenediamine (DMPD) assays and represented as trolox equivalent antioxidant capacity (TEAC) and ascorbic acid equivalent antioxidant capacity (AEAC). Antioxidant capacity ranged from 121.02 ± 6.05 to 1567.28 ± 78.36 µM trolox equivalent antioxidant capacity and 56.62 ± 2.83 to 972.48 ± 48.62 µM ascorbic acid equivalent antioxidant capacity. Roots showed higher yields of illustrated biochemical parameters, however fresh fruit pulp was found a chief source of minerals. Gas chromatography-mass spectroscopic analysis revealed the presence of a vast array of phytoconstituents associated with different plant parts. The present study revealed the amounts of minerals and diverse phytoconstituents in various parts of and confirmed its medicinal and nutritional implications.
该研究旨在评估某植物不同部位的植物化学物质、矿物质的提取效率、检测和定量以及抗氧化能力。采用连续振荡提取、蒸汽浴辅助提取、超声提取和微波辅助提取等不同时间间隔的方法来提取酚类、黄酮类和抗氧化剂。初步筛选发现除了碳水化合物和淀粉外,还存在大量的代谢物。结果发现,蒸汽浴辅助提取10分钟最适合提取酚类物质(每克干重含46.02±2.30毫克没食子酸当量和48.57±2.42毫克单宁酸当量)和黄酮类物质(每克干重含35.26±1.61毫克槲皮素当量和51.60±2.58毫克鞣花酸当量)。作为佐证,反相高效液相色谱-二极管阵列检测器证实了七种具有重要药用价值的酚酸的存在。通过1,1-二苯基-2-苦基肼(DPPH)、铁还原抗氧化能力(FRAP)、2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)清除能力(ABTS)和N,N-二甲基对苯二胺(DMPD)测定法来测量抗氧化能力,并以Trolox当量抗氧化能力(TEAC)和抗坏血酸当量抗氧化能力(AEAC)表示。抗氧化能力范围为121.02±6.05至1567.28±78.36微摩尔Trolox当量抗氧化能力以及56.62±2.83至972.48±48.62微摩尔抗坏血酸当量抗氧化能力。根部所呈现的生化参数产量较高,然而新鲜果肉是矿物质的主要来源。气相色谱-质谱分析显示不同植物部位存在大量的植物成分。本研究揭示了该植物各部位矿物质和多种植物成分的含量,并证实了其药用和营养意义。