Department of Pediatric Surgery, Kocaeli University Medical Faculty, Umuttepe, 41380, Kocaeli, Turkey.
Republic of Turkey Ministry of Health, Ankara, Turkey.
Esophagus. 2019 Oct;16(4):352-361. doi: 10.1007/s10388-019-00671-2. Epub 2019 Apr 11.
BACKGROUND/PURPOSE: The inflammatory response that follows the caustic burns results in fibrosis on the esophageal wall leading to esophageal stricture, dysphagia, and malnutrition. The controversy over the use of corticosteroids warrants alternative therapeutic interventions. We investigated the effect of extracts from St. John's wort (SJW) with known wound-healing activity on stricture formation in rat esophageal injury models.
Five experimental groups were involved: sham group with no injury, control group with injury without treatment, and three different treatment groups (methylprednisolone, SJW extract, and combination of the two). Histopathological examination of esophageal damage and collagen accumulation, stenosis index, and tissue hydroxyproline levels were used to assess stricture and the effect of treatments.
There was a significant weight loss in all groups except for those without injury and those treated with SJW extract, the latter gained weight albeit not significant. Stenosis index was increased in all groups compared to sham but not significantly in those treated with SJW extract. Histopathological and biochemical analyses produced mixed results.
Some of the experimental indicators such as weight gain and stenosis index suggested the treatment of esophageal injury models using extracts of St. John's wort effective while other histopathological indicators show no significant benefit.
背景/目的：腐蚀性烧伤后引发的炎症反应会导致食管壁纤维化，进而导致食管狭窄、吞咽困难和营养不良。皮质类固醇的使用争议需要替代治疗干预。我们研究了具有已知愈合活性的贯叶连翘（SJW）提取物对大鼠食管损伤模型中狭窄形成的影响。
涉及五个实验组：无损伤的假手术组、无治疗的损伤对照组，以及三种不同的治疗组（甲泼尼龙、SJW 提取物和两者的组合）。通过组织学检查评估食管损伤和胶原积累、狭窄指数和组织羟脯氨酸水平，以评估狭窄和治疗效果。
除未受伤组和 SJW 提取物治疗组外，所有组的体重均明显减轻，后者虽然体重增加，但无统计学意义。与假手术组相比，所有组的狭窄指数均升高，但 SJW 提取物治疗组无显著升高。组织学和生化分析结果不一致。
一些实验指标，如体重增加和狭窄指数，表明使用贯叶连翘提取物治疗食管损伤模型有效，而其他组织病理学指标则没有明显的益处。
