Inoue Kota, Tsujio Masashi, Matsubayashi Makoto, Inoue Ryota, Hatai Hitoshi, Andoh Masako, Abe Keisuke, Matsui Toshihiro, Matsuo Tomohide
Laboratory of Parasitology, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, 890-0065, Japan.
Laboratory of Anatomy, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, 890-0065, Japan.
Acta Parasitol. 2019 Jun;64(2):418-422. doi: 10.2478/s11686-019-00052-w. Epub 2019 Apr 11.
Murine Eimeria spp. have been used as effective experimental models of disease instead of large mammalian hosts such as cattle. We here examine drug susceptibility of the uncharacterized murine intestinal protozoan parasite, Eimeria krijgsmanni.
The effectiveness of different treatments against infection of E. krijgsmanni was examined for suppression of oocyst shedding: ST mixture ST mixture, pyrimethamine, Ektecin and toltrazuril.
ST mixture and pyrimethamine did not suppress oocyst shedding effectively. Although therapeutic efficacy of Ektecin was demonstrated, the dose required was larger than that for cattle and chickens. Oocyst shedding was only completely suppressed completely by continuous administration of toltrazuril. Furthermore, it was confirmed through morphological examination that early developmental stage zoites appeared in host epithelial cells during and following treatment by toltrazuril, and toltrazuril could not eliminate residual zoites in epithelial cells.
E. krijgsmanni may be relatively resistant to these anti-coccidian agents and might therefore have different characteristics that differ from other coccidia with regard to drug susceptibility.
鼠艾美耳球虫已被用作有效的疾病实验模型，而非像牛这样的大型哺乳动物宿主。我们在此研究未被描述的鼠肠道原生动物寄生虫克氏艾美耳球虫的药物敏感性。
检测了不同治疗方法对克氏艾美耳球虫感染的有效性，以抑制卵囊排出：ST混合物、乙胺嘧啶、埃克替尼和托曲珠利。
ST混合物和乙胺嘧啶不能有效抑制卵囊排出。虽然已证明埃克替尼有治疗效果，但其所需剂量比牛和鸡的剂量大。仅通过持续施用托曲珠利可完全抑制卵囊排出。此外，通过形态学检查证实，在托曲珠利治疗期间及之后，宿主上皮细胞中出现了早期发育阶段的子孢子，且托曲珠利无法消除上皮细胞中的残留子孢子。
克氏艾美耳球虫可能对这些抗球虫药具有相对抗性，因此在药物敏感性方面可能具有与其他球虫不同的特征。
