Abu-Bonsrah Kwaku Dad, Viventi Serena, Newgreen Donald F, Dottori Mirella
Department of Paedriatrics, University of Melbourne, Parkville, VIC, Australia.
Murdoch Childrens Research Institute, Parkville, VIC, Australia.
Methods Mol Biol. 2019;1976:37-47. doi: 10.1007/978-1-4939-9412-0_3.
There are a vast range of diseases and disorders that are neurocristopathic in origin, including Hirschsprung's disease, pheochromocytoma, familial dysautonomia, craniofacial disorders, and melanomas. Having a source of human neural crest cells is highly valuable for investigating potential treatments for such diseases. This chapter describes a robust and well-characterized protocol for deriving neural crest from human pluripotent stem cells (hPSCs), which can then be differentiated to neuronal and non-neuronal lineages. The protocol is adapted to suit hPSC maintenance as a monolayer bulk culture or as manual-passaged colonies, which makes it widely applicable to researchers that may use different systems for hPSC maintenance.
有多种起源于神经嵴病变的疾病和病症，包括先天性巨结肠、嗜铬细胞瘤、家族性自主神经功能异常、颅面疾病和黑色素瘤。拥有人类神经嵴细胞来源对于研究此类疾病的潜在治疗方法非常有价值。本章描述了一种从人多能干细胞（hPSC）中获取神经嵴的强大且特征明确的方案，然后可将其分化为神经元和非神经元谱系。该方案适用于将hPSC维持为单层批量培养或手动传代的集落，这使其广泛适用于可能使用不同系统维持hPSC的研究人员。
