神经嵴细胞迁移的体内和体外定量分析

In Vivo and In Vitro Quantitative Analysis of Neural Crest Cell Migration.

作者信息

Barriga Elias H, Shellard Adam, Mayor Roberto

机构信息

Department of Cell and Developmental Biology, University College London, London, UK.

London Centre for Nanotechnology, University College London, London, UK.

出版信息

Methods Mol Biol. 2019;1976:135-152. doi: 10.1007/978-1-4939-9412-0_11.

DOI:10.1007/978-1-4939-9412-0_11

PMID:30977071

Abstract

The neural crest is an embryonic cell population induced at the border of the neural plate from where it delaminates and migrates long distances across the embryo. Due to its extraordinary migratory capabilities, the neural crest has become a powerful system to study cellular and molecular aspects of collective and single cell migration both in vivo and in vitro. Here we provide detailed protocols used to perform quantitative analysis of molecular and cellular aspects of Xenopus laevis neural crest cell migration, both in vivo and in vitro.

摘要

神经嵴是一种胚胎细胞群，在神经板边界处被诱导产生，从这里它分层并在胚胎中远距离迁移。由于其非凡的迁移能力，神经嵴已成为一个强大的系统，用于研究体内和体外集体细胞迁移和单细胞迁移的细胞和分子方面。在这里，我们提供了用于对非洲爪蟾神经嵴细胞在体内和体外迁移的分子和细胞方面进行定量分析的详细方案。