State Key Laboratory of Mycology, Institute of Microbiology , Chinese Academy of Sciences , Beijing 100101 , People's Republic of China.
Savaid Medical School , University of Chinese Academy of Sciences , Beijing 100049 , People's Republic of China.
Org Lett. 2019 May 3;21(9):3252-3256. doi: 10.1021/acs.orglett.9b00984. Epub 2019 Apr 12.
A rare fungal hopane-type triterpenoid glycoside fumihopaside A was identified by genome mining in combination with HPLC-MS/MS in Aspergillus fumigatus. Combining genetic deletions in A. fumigatus with heterologous reconstitutions in Aspergillus nidulans of the afum gene cluster, we identified one fungal squalene hopane cyclase AfumA charging the cyclization of the hopene skeleton, one cytochrome P450, and one UDP-glycosyltransferase. Bioassays indicated that fumihopaside A plays important roles in protecting A. fumigatus against heat or ultraviolet stress.
通过在烟曲霉中进行基因组挖掘并结合 HPLC-MS/MS，鉴定出一种罕见的真菌藿烷型三萜糖苷 fumihopaside A。通过烟曲霉中的基因缺失和在构巢曲霉中的异源重建 afum 基因簇，我们鉴定出一个真菌角鲨烯藿烷环化酶 AfumA 负责藿烯骨架的环化、一个细胞色素 P450 和一个 UDP-糖基转移酶。生物测定表明，fumihopaside A 在保护烟曲霉免受热或紫外线应激方面发挥重要作用。
