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成纤维细胞影响杯状细胞对炎症细胞因子和黏液破坏剂暴露后乳酸杆菌的反应。

Fibroblasts Impact Goblet Cell Responses to Lactic Acid Bacteria After Exposure to Inflammatory Cytokines and Mucus Disruptors.

机构信息

Immunoendocrinology, Division of Medical Biology, Department of Pathology and Medical Biology, University of Groningen and University Medical Center Groningen, 9700, RB, Groningen, The Netherlands.

School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.

出版信息

Mol Nutr Food Res. 2019 Jun;63(12):e1801427. doi: 10.1002/mnfr.201801427. Epub 2019 Apr 22.

DOI:10.1002/mnfr.201801427
PMID:30977971
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6618108/
Abstract

SCOPE

Mucus produced by goblet cells contributes to gut barrier function. Lactic acid bacteria (LAB) have been shown to impact mucus production. It is not completely known whether mucus production is influenced by the abundantly present fibroblasts in the intestine.

METHODS AND RESULTS

The influence of fibroblasts on mucus-related genes including mucin-2 (MUC2), trefoil factor 3 (TFF3), resistin-like molecule β (RETNLB), carbohydrate sulfotransferase 5 (CHST5), and galactose-3-O-sulfotransferase 2 (GAL3ST2) is examined after co-culture of LS174T-goblet cells and CCD-18Co colonic fibroblasts in the presence and absence of LAB-strains known to impact mucus function. This is also tested after exposure to TNF-α, IL-13, or the mucin synthesis inhibitor tunicamycin (Tm). Effects of fibroblasts are treatment duration- and bacterial species-dependent under homeostatic conditions. During TNF-α challenge, fibroblasts reverse Lactobacillus (L.) rhamnosus CCFM237-elicited declined TFF3 expression. After IL-13 exposure, L. rhamnosus CCFM237 and L. fermentum CCFM787 attenuate enhanced TFF3 and RETNLB expression, respectively, only in the presence of fibroblasts. LAB has no effects on Tm-induced decreased expression of goblet cell-related genes regardless of the presence of fibroblasts.

CONCLUSION

It is demonstrated that goblet cell-fibroblast crosstalk impacts mucus synthesis and influences the effects of LAB on goblet cell-related genes. Effects are LAB-species and stressor dependent.

摘要

范围

杯状细胞产生的黏液有助于肠道屏障功能。已证实乳酸菌（LAB）会影响黏液的产生。目前尚不完全清楚肠道中大量存在的成纤维细胞是否会影响黏液的产生。

方法和结果

在存在和不存在已知影响黏液功能的 LAB 菌株的情况下，LS174T-杯状细胞和 CCD-18Co 结肠成纤维细胞共培养后，研究了成纤维细胞对包括黏蛋白-2（MUC2）、三叶因子 3（TFF3）、抵抗素样分子β（RETNLB）、碳水化合物磺基转移酶 5（CHST5）和半乳糖-3-O-磺基转移酶 2（GAL3ST2）在内的与黏液相关基因的影响。在 TNF-α、IL-13 或黏液合成抑制剂乳胞素（Tm）暴露后也进行了测试。在稳态条件下，成纤维细胞的作用取决于处理持续时间和细菌种类。在 TNF-α 挑战期间，成纤维细胞逆转了 Lactobacillus（L.） rhamnosus CCFM237 引起的 TFF3 表达下降。在 IL-13 暴露后，L. rhamnosus CCFM237 和 L. fermentum CCFM787 分别在存在成纤维细胞的情况下减弱了增强的 TFF3 和 RETNLB 表达。无论是否存在成纤维细胞，LAB 对 Tm 诱导的杯状细胞相关基因表达下降均无影响。

结论

研究表明，杯状细胞和成纤维细胞的相互作用会影响黏液的合成，并影响 LAB 对杯状细胞相关基因的作用。作用取决于 LAB 种类和应激源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5289/6618108/e275b359a0a2/MNFR-63-na-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5289/6618108/7f748b420336/MNFR-63-na-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5289/6618108/b3b0a98f0f9e/MNFR-63-na-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5289/6618108/edb25fd61254/MNFR-63-na-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5289/6618108/d4331ae09116/MNFR-63-na-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5289/6618108/521b84d41c39/MNFR-63-na-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5289/6618108/e275b359a0a2/MNFR-63-na-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5289/6618108/7f748b420336/MNFR-63-na-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5289/6618108/c0a5967a90d6/MNFR-63-na-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5289/6618108/3e46e31273c4/MNFR-63-na-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5289/6618108/b3b0a98f0f9e/MNFR-63-na-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5289/6618108/edb25fd61254/MNFR-63-na-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5289/6618108/d4331ae09116/MNFR-63-na-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5289/6618108/521b84d41c39/MNFR-63-na-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5289/6618108/e275b359a0a2/MNFR-63-na-g009.jpg

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Butyrate Supplementation at High Concentrations Alters Enteric Bacterial Communities and Reduces Intestinal Inflammation in Mice Infected with .
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